Expression and Purification of Mini G Proteins from Escherichia coli

Heterotrimeric G proteins modulate intracellular signalling by transducing information from cell surface G protein-coupled receptors (GPCRs) to cytoplasmic effector proteins. Structural and functional characterisation of GPCR–G protein complexes is important to fully decipher the mechanism of signal...

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Bibliographic Details
Main Authors: Byron Carpenter, Christopher Tate
Format: Article
Language:English
Published: Bio-protocol LLC 2017-04-01
Series:Bio-Protocol
Online Access:https://bio-protocol.org/en/bpdetail?id=2235&type=0