Hypoxic Three-Dimensional Scaffold-Free Aggregate Cultivation of Mesenchymal Stem Cells in a Stirred Tank Reactor

Hypoxic Three-Dimensional Scaffold-Free Aggregate Cultivation of Mesenchymal Stem Cells in a Stirred Tank Reactor

Extensive expansion of mesenchymal stem cells (MSCs) for cell-based therapies remains challenging since long-term cultivation and excessive passaging in two-dimensional conditions result in a loss of essential stem cell properties. Indeed, low survival rate of cells, alteration of surface marker pro...

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Bibliographic Details
Main Authors: Dominik Egger, Ivo Schwedhelm, Jan Hansmann, Cornelia Kasper
Format: Article
Language:English
Published: MDPI AG 2017-05-01
Series:Bioengineering
Subjects:
mesenchymal stem cells
scaffold-free
aggregate cultivation
stirred tank reactor
dynamic cultivation
hypoxia
stemness
computational fluid dynamics
Online Access:http://www.mdpi.com/2306-5354/4/2/47

Internet

http://www.mdpi.com/2306-5354/4/2/47

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