Point mutation of V252 in neomycin C epimerase enlarges substrate-binding pocket and improves neomycin B accumulation in Streptomyces fradiae

Point mutation of V252 in neomycin C epimerase enlarges substrate-binding pocket and improves neomycin B accumulation in Streptomyces fradiae

Abstract Neomycin, an aminoglycoside antibiotic with broad-spectrum antibacterial resistance, is widely used in pharmaceutical and agricultural fields. However, separation and purification of neomycin B as an active substance from Streptomyces fradiae are complicated. Although NeoN can catalyze conv...

Full description

Bibliographic Details
Main Authors: Xiangfei Li, Fei Yu, Fang Wang, Sang Wang, Rumeng Han, Yihan Cheng, Ming Zhao, Junfeng Sun, Zhenglian Xue
Format: Article
Language:English
Published: SpringerOpen 2022-12-01
Series:Bioresources and Bioprocessing
Subjects:
S. fradiae
Neomycin
NeoN
Catalytic mechanism
Online Access:https://doi.org/10.1186/s40643-022-00613-4

Internet

https://doi.org/10.1186/s40643-022-00613-4

Similar Items