Rapid in-EPON CLEM: Combining fast and efficient labeling of self-labeling enzyme tags with EM-resistant Janelia Fluor dyes and StayGold

Rapid in-EPON CLEM: Combining fast and efficient labeling of self-labeling enzyme tags with EM-resistant Janelia Fluor dyes and StayGold

Correlative light and electron microscopy (CLEM) combines light microscopy (LM) of fluorescent samples to ultrastructural analyses by electron microscopy (EM). Pre-embedding CLEM often suffers from inaccurate correlation between LM and EM modalities. Post-embedding CLEM enables precise registration...

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Bibliographic Details
Main Authors: Rico Franzkoch, Sabrina Wilkening, Viktoria Liss, Michael Holtmannspötter, Rainer Kurre, Olympia E. Psathaki, Michael Hensel
Format: Article
Language:English
Published: Elsevier 2024-04-01
Series:Heliyon
Subjects:
In-resin correlative light and electron microscopy
Post-embedding CLEM
Self-labeling enzyme tags
Janelia fluor dyes
HaloTag
Online Access:http://www.sciencedirect.com/science/article/pii/S2405844024040866

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http://www.sciencedirect.com/science/article/pii/S2405844024040866

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