Isolation of Primary Mouse Pulmonary Microvascular Endothelial Cells and Generation of an Immortalized Cell Line to Obtain Sufficient Extracellular Vesicles

Isolation of Primary Mouse Pulmonary Microvascular Endothelial Cells and Generation of an Immortalized Cell Line to Obtain Sufficient Extracellular Vesicles

Pulmonary microvascular endothelial cells (PMECs) and the extracellular vesicles (EVs) derived from PMECs participate in maintaining pulmonary homeostasis and mediating the inflammatory response. However, obtaining a high-purity population of PMECs and their EVs from mouse is still notoriously diffi...

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Bibliographic Details
Main Authors: Xu Liu, Feiping Xia, Xiao Wu, Ying Tang, Lu Wang, Qin Sun, Ming Xue, Wei Chang, Ling Liu, Fengmei Guo, Yi Yang, Haibo Qiu
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-12-01
Series:Frontiers in Immunology
Subjects:
pulmonary microvascular endothelial cells
immortalized cell line
extracellular vesicles
cell culture
pulmonary inflammation
Online Access:https://www.frontiersin.org/articles/10.3389/fimmu.2021.759176/full

Internet

https://www.frontiersin.org/articles/10.3389/fimmu.2021.759176/full

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