Targeting Mosquitoes through Generation of an Insecticidal RNAi Yeast Strain Using Cas-CLOVER and Super PiggyBac Engineering in <i>Saccharomyces cerevisiae</i>

Targeting Mosquitoes through Generation of an Insecticidal RNAi Yeast Strain Using Cas-CLOVER and Super PiggyBac Engineering in <i>Saccharomyces cerevisiae</i>

The global deployment of RNAi yeast insecticides involves transitioning from the use of laboratory yeast strains to more robust strains that are suitable for scaled fermentation. In this investigation, the RNA-guided Cas-CLOVER system was used in combination with Piggybac transposase to produce robu...

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Bibliographic Details
Main Authors: Corey Brizzee, Keshava Mysore, Teresia M. Njoroge, Seth McConnell, Majidah Hamid-Adiamoh, Akilah T. M. Stewart, J. Tyler Kinder, Jack Crawford, Molly Duman-Scheel
Format: Article
Language:English
Published: MDPI AG 2023-10-01
Series:Journal of Fungi
Subjects:
<i>Saccharomyces cerevisiae</i>
engineering
synthetic biology
mosquitoes
shRNA
<i>Aedes</i>
Online Access:https://www.mdpi.com/2309-608X/9/11/1056

Internet

https://www.mdpi.com/2309-608X/9/11/1056

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