Cryopreservation of Brazilian green dwarf coconut plumules by droplet-vitrification
ABSTRACT: This study evaluated the effect of vitrification solutions and exposure time on the cryopreservation of Brazilian green dwarf coconut plumules (BGD) using the droplet vitrification technique. Explants were excised from BGD mature fruits from the Active Germplasm Bank of Embrapa Tabuleiros...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Santa Maria
2019-12-01
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| Series: | Ciência Rural |
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| Online Access: | http://www.scielo.br/pdf/cr/v50n1/1678-4596-cr-50-01-e20190020.pdf |
| _version_ | 1818743604721483776 |
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| author | Ana da Silva Lédo Fernanda Vieira Santana Annie Carolina Araújo de Oliveira Leila Albuquerque Resende de Oliveira Ana Veruska Cruz da Silva |
| author_facet | Ana da Silva Lédo Fernanda Vieira Santana Annie Carolina Araújo de Oliveira Leila Albuquerque Resende de Oliveira Ana Veruska Cruz da Silva |
| author_sort | Ana da Silva Lédo |
| collection | DOAJ |
| description | ABSTRACT: This study evaluated the effect of vitrification solutions and exposure time on the cryopreservation of Brazilian green dwarf coconut plumules (BGD) using the droplet vitrification technique. Explants were excised from BGD mature fruits from the Active Germplasm Bank of Embrapa Tabuleiros Costeiros, Sergipe, Brazil. Firstly, embryos were disinfected, and after excision, plumules were pre-cultivated for 72 hours in Y3 + 0.6 M sucrose + 2.2 g L-1 Gelrite® culture medium. Plumules were exposed to PVS2 and PVS3 solutions for 15 and 30 minutes and rapidly immersed in liquid nitrogen (-196 ºC). After cryopreservation, they were thawed in culture medium solution (Y3 + 1.2 M sucrose) and cultured in regeneration medium. The experimental design was completely randomized in a 2x2 factorial scheme (vitrification solutions per exposure times), with five replicates per treatment. Data were compared by the Tukey’s test at 5% probability. Significant differences were observed in the callogenesis percentage for the solutions x exposure time interaction for non-cryopreserved cultures (-NL) and for exposure time after cryopreservation (+NL). PVS2 and PVS3 combined with 15 minutes of exposure promoted the highest callus formation (70 and 100%, respectively) in control cultures. The exposure time of 30 min, regardless of vitrification solution, resulted in 30% embryogenic callus formation after cryopreservation. These results contributed to the long-term conservation of coconut palm. |
| first_indexed | 2024-12-18T02:31:03Z |
| format | Article |
| id | doaj.art-e5c273e4c7f3473fba7e264d8e145c98 |
| institution | Directory Open Access Journal |
| issn | 1678-4596 |
| language | English |
| last_indexed | 2024-12-18T02:31:03Z |
| publishDate | 2019-12-01 |
| publisher | Universidade Federal de Santa Maria |
| record_format | Article |
| series | Ciência Rural |
| spelling | doaj.art-e5c273e4c7f3473fba7e264d8e145c982022-12-21T21:23:54ZengUniversidade Federal de Santa MariaCiência Rural1678-45962019-12-0150110.1590/0103-8478cr20190020Cryopreservation of Brazilian green dwarf coconut plumules by droplet-vitrificationAna da Silva Lédohttps://orcid.org/0000-0002-4353-4788Fernanda Vieira Santanahttps://orcid.org/0000-0003-3627-1251Annie Carolina Araújo de Oliveirahttps://orcid.org/0000-0001-6916-3586Leila Albuquerque Resende de Oliveirahttps://orcid.org/0000-0002-6016-7908Ana Veruska Cruz da SilvaABSTRACT: This study evaluated the effect of vitrification solutions and exposure time on the cryopreservation of Brazilian green dwarf coconut plumules (BGD) using the droplet vitrification technique. Explants were excised from BGD mature fruits from the Active Germplasm Bank of Embrapa Tabuleiros Costeiros, Sergipe, Brazil. Firstly, embryos were disinfected, and after excision, plumules were pre-cultivated for 72 hours in Y3 + 0.6 M sucrose + 2.2 g L-1 Gelrite® culture medium. Plumules were exposed to PVS2 and PVS3 solutions for 15 and 30 minutes and rapidly immersed in liquid nitrogen (-196 ºC). After cryopreservation, they were thawed in culture medium solution (Y3 + 1.2 M sucrose) and cultured in regeneration medium. The experimental design was completely randomized in a 2x2 factorial scheme (vitrification solutions per exposure times), with five replicates per treatment. Data were compared by the Tukey’s test at 5% probability. Significant differences were observed in the callogenesis percentage for the solutions x exposure time interaction for non-cryopreserved cultures (-NL) and for exposure time after cryopreservation (+NL). PVS2 and PVS3 combined with 15 minutes of exposure promoted the highest callus formation (70 and 100%, respectively) in control cultures. The exposure time of 30 min, regardless of vitrification solution, resulted in 30% embryogenic callus formation after cryopreservation. These results contributed to the long-term conservation of coconut palm.http://www.scielo.br/pdf/cr/v50n1/1678-4596-cr-50-01-e20190020.pdfCocos nucifera L.cryoprotectionPVS2PVS3 |
| spellingShingle | Ana da Silva Lédo Fernanda Vieira Santana Annie Carolina Araújo de Oliveira Leila Albuquerque Resende de Oliveira Ana Veruska Cruz da Silva Cryopreservation of Brazilian green dwarf coconut plumules by droplet-vitrification Ciência Rural Cocos nucifera L. cryoprotection PVS2 PVS3 |
| title | Cryopreservation of Brazilian green dwarf coconut plumules by droplet-vitrification |
| title_full | Cryopreservation of Brazilian green dwarf coconut plumules by droplet-vitrification |
| title_fullStr | Cryopreservation of Brazilian green dwarf coconut plumules by droplet-vitrification |
| title_full_unstemmed | Cryopreservation of Brazilian green dwarf coconut plumules by droplet-vitrification |
| title_short | Cryopreservation of Brazilian green dwarf coconut plumules by droplet-vitrification |
| title_sort | cryopreservation of brazilian green dwarf coconut plumules by droplet vitrification |
| topic | Cocos nucifera L. cryoprotection PVS2 PVS3 |
| url | http://www.scielo.br/pdf/cr/v50n1/1678-4596-cr-50-01-e20190020.pdf |
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