Microbial Cell Factory of Baccatin III Preparation in Escherichia coli by Increasing DBAT Thermostability and in vivo Acetyl-CoA Supply

Microbial Cell Factory of Baccatin III Preparation in Escherichia coli by Increasing DBAT Thermostability and in vivo Acetyl-CoA Supply

Given the rapid development of genome mining in this decade, the substrate channel of paclitaxel might be identified in the near future. A robust microbial cell factory with gene dbat, encoding a key rate-limiting enzyme 10-deacetylbaccatin III-10-O-transferase (DBAT) in paclitaxel biosynthesis to s...

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Bibliographic Details
Main Authors: Jia-jun Huang, Tao Wei, Zhi-wei Ye, Qian-wang Zheng, Bing-hua Jiang, Wen-feng Han, An-qi Ye, Pei-yun Han, Li-qiong Guo, Jun-fang Lin
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-01-01
Series:Frontiers in Microbiology
Subjects:
baccatin III
10-deacetylbaccatin III-10-O-transferase
gene integration
semi-rational design
thermostability
acetyl-CoA supplement
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2021.803490/full

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https://www.frontiersin.org/articles/10.3389/fmicb.2021.803490/full

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