Combining Mutations That Inhibit Two Distinct Steps of the ATP Hydrolysis Cycle Restores Wild-Type Function in the Lipopolysaccharide Transporter and Shows that ATP Binding Triggers Transport

ABSTRACT ATP-binding cassette (ABC) transporters constitute a large family of proteins present in all domains of life. They are powered by dynamic ATPases that harness energy from binding and hydrolyzing ATP through a cycle that involves the closing and reopening of their two ATP-binding domains. Th...

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Bibliographic Details
Main Authors: Brent W. Simpson, Karanbir S. Pahil, Tristan W. Owens, Emily A. Lundstedt, Rebecca M. Davis, Daniel Kahne, Natividad Ruiz
Format: Article
Language:English
Published: American Society for Microbiology 2019-08-01
Series:mBio
Subjects:
Online Access:https://journals.asm.org/doi/10.1128/mBio.01931-19