CRISPR/Cas9-mediated base-editing enables a chain reaction through sequential repair of sgRNA scaffold mutations

CRISPR/Cas9-mediated base-editing enables a chain reaction through sequential repair of sgRNA scaffold mutations

Abstract Cell behavior is controlled by complex gene regulatory networks. Although studies have uncovered diverse roles of individual genes, it has been challenging to record or control sequential genetic events in living cells. In this study, we designed two cellular chain reaction systems that ena...

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Bibliographic Details
Main Authors: Tsuyoshi Fukushima, Yosuke Tanaka, Keito Adachi, Nanami Masuyama, Akiho Tsuchiya, Shuhei Asada, Soh Ishiguro, Hideto Mori, Motoaki Seki, Nozomu Yachie, Susumu Goyama, Toshio Kitamura
Format: Article
Language:English
Published: Nature Portfolio 2021-12-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-02986-6

Internet

https://doi.org/10.1038/s41598-021-02986-6

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