Signal Enhancement in Oriented Immunosorbent Assays: A Balance between Accessibility of Antigen Binding Sites and Avidity

Signal Enhancement in Oriented Immunosorbent Assays: A Balance between Accessibility of Antigen Binding Sites and Avidity

The sensitivity of immunoassays was reported to be increased by the orientation of antibodies. We investigated how the size and valence of antigens and orientation and valence of antibodies contribute to the analytical sensitivity of ELISA. Antigens differing in size and number of epitopes were comp...

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Bibliographic Details
Main Authors: Vanessa Susini, Vanna Fierabracci, Gaia Barria, Lisa Dodoli, Laura Caponi, Aldo Paolicchi, Maria Franzini
Format: Article
Language:English
Published: MDPI AG 2021-12-01
Series:Biosensors
Subjects:
immunoassay
oriented binding
steric hindrance
antibody avidity
reduced IgG
half-fragment antibodies
Online Access:https://www.mdpi.com/2079-6374/11/12/493

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https://www.mdpi.com/2079-6374/11/12/493

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