Differential and Common Signatures of miRNA Expression and Methylation in Childhood Central Nervous System Malignancies: An Experimental and Computational Approach
Epigenetic modifications are considered of utmost significance for tumor ontogenesis and progression. Especially, it has been found that miRNA expression, as well as DNA methylation plays a significant role in central nervous system tumors during childhood. A total of 49 resected brain tumors from c...
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| Format: | Article |
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MDPI AG
2021-10-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/13/21/5491 |
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| author | George I. Lambrou Myrto Poulou Krinio Giannikou Marios Themistocleous Apostolos Zaravinos Maria Braoudaki |
| author_facet | George I. Lambrou Myrto Poulou Krinio Giannikou Marios Themistocleous Apostolos Zaravinos Maria Braoudaki |
| author_sort | George I. Lambrou |
| collection | DOAJ |
| description | Epigenetic modifications are considered of utmost significance for tumor ontogenesis and progression. Especially, it has been found that miRNA expression, as well as DNA methylation plays a significant role in central nervous system tumors during childhood. A total of 49 resected brain tumors from children were used for further analysis. DNA methylation was identified with methylation-specific MLPA and, in particular, for the tumor suppressor genes CASP8, RASSF1, MGMT, MSH6, GATA5, ATM1, TP53, and CADM1. miRNAs were identified with microarray screening, as well as selected samples, were tested for their mRNA expression levels. CASP8, RASSF1 were the most frequently methylated genes in all tumor samples. Simultaneous methylation of genes manifested significant results with respect to tumor staging, tumor type, and the differentiation of tumor and control samples. There was no significant dependence observed with the methylation of one gene promoter, rather with the simultaneous presence of all detected methylated genes’ promoters. miRNA expression was found to be correlated to gene methylation. Epigenetic regulation appears to be of major importance in tumor progression and pathophysiology, making it an imperative field of study. |
| first_indexed | 2024-03-10T06:06:03Z |
| format | Article |
| id | doaj.art-e6699fccef764898a623cf366ab6f6bb |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T06:06:03Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-e6699fccef764898a623cf366ab6f6bb2023-11-22T20:36:03ZengMDPI AGCancers2072-66942021-10-011321549110.3390/cancers13215491Differential and Common Signatures of miRNA Expression and Methylation in Childhood Central Nervous System Malignancies: An Experimental and Computational ApproachGeorge I. Lambrou0Myrto Poulou1Krinio Giannikou2Marios Themistocleous3Apostolos Zaravinos4Maria Braoudaki5Choremeio Research Laboratory, First Department of Pediatrics, National and Kapodistrian University of Athens, 11527 Athens, GreeceDepartment of Medical Genetics, Medical School, National and Kapodistrian University of Athens, 15772 Athens, GreeceCancer Genetics Laboratory, Division of Pulmonary and Critical Care Medicine and of Genetics, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA 02115, USADepartment of Neurosurgery, “Aghia Sofia” Children’s Hospital, 11527 Athens, GreeceDepartment of Life Sciences, School of Sciences, European University Cyprus, Nicosia 2404, CyprusDepartment of Life and Environmental Sciences, School of Life and Health Sciences, University of Hertfordshire, Hertfordshire AL10 9AB, UKEpigenetic modifications are considered of utmost significance for tumor ontogenesis and progression. Especially, it has been found that miRNA expression, as well as DNA methylation plays a significant role in central nervous system tumors during childhood. A total of 49 resected brain tumors from children were used for further analysis. DNA methylation was identified with methylation-specific MLPA and, in particular, for the tumor suppressor genes CASP8, RASSF1, MGMT, MSH6, GATA5, ATM1, TP53, and CADM1. miRNAs were identified with microarray screening, as well as selected samples, were tested for their mRNA expression levels. CASP8, RASSF1 were the most frequently methylated genes in all tumor samples. Simultaneous methylation of genes manifested significant results with respect to tumor staging, tumor type, and the differentiation of tumor and control samples. There was no significant dependence observed with the methylation of one gene promoter, rather with the simultaneous presence of all detected methylated genes’ promoters. miRNA expression was found to be correlated to gene methylation. Epigenetic regulation appears to be of major importance in tumor progression and pathophysiology, making it an imperative field of study.https://www.mdpi.com/2072-6694/13/21/5491methylationchildhood CNS tumorsmiRNAmRNAmicroarray |
| spellingShingle | George I. Lambrou Myrto Poulou Krinio Giannikou Marios Themistocleous Apostolos Zaravinos Maria Braoudaki Differential and Common Signatures of miRNA Expression and Methylation in Childhood Central Nervous System Malignancies: An Experimental and Computational Approach Cancers methylation childhood CNS tumors miRNA mRNA microarray |
| title | Differential and Common Signatures of miRNA Expression and Methylation in Childhood Central Nervous System Malignancies: An Experimental and Computational Approach |
| title_full | Differential and Common Signatures of miRNA Expression and Methylation in Childhood Central Nervous System Malignancies: An Experimental and Computational Approach |
| title_fullStr | Differential and Common Signatures of miRNA Expression and Methylation in Childhood Central Nervous System Malignancies: An Experimental and Computational Approach |
| title_full_unstemmed | Differential and Common Signatures of miRNA Expression and Methylation in Childhood Central Nervous System Malignancies: An Experimental and Computational Approach |
| title_short | Differential and Common Signatures of miRNA Expression and Methylation in Childhood Central Nervous System Malignancies: An Experimental and Computational Approach |
| title_sort | differential and common signatures of mirna expression and methylation in childhood central nervous system malignancies an experimental and computational approach |
| topic | methylation childhood CNS tumors miRNA mRNA microarray |
| url | https://www.mdpi.com/2072-6694/13/21/5491 |
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