Reproducible proteomics sample preparation for single FFPE tissue slices using acid-labile surfactant and direct trypsinization

Reproducible proteomics sample preparation for single FFPE tissue slices using acid-labile surfactant and direct trypsinization

Abstract Background Proteomic analyses of clinical specimens often rely on human tissues preserved through formalin-fixation and paraffin embedding (FFPE). Minimal sample consumption is the key to preserve the integrity of pathological archives but also to deal with minimal invasive core biopsies. T...

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Bibliographic Details
Main Authors: Melanie Christine Föll, Matthias Fahrner, Victor Oginga Oria, Markus Kühs, Martin Lothar Biniossek, Martin Werner, Peter Bronsert, Oliver Schilling
Format: Article
Language:English
Published: BMC 2018-03-01
Series:Clinical Proteomics
Subjects:
Mass spectrometry
Proteomics
Label-free quantitation
FFPE
Archival tissue
FASP
Online Access:http://link.springer.com/article/10.1186/s12014-018-9188-y

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http://link.springer.com/article/10.1186/s12014-018-9188-y

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