An improved protocol for intact chloroplasts and cpDNA isolation in conifers.

An improved protocol for intact chloroplasts and cpDNA isolation in conifers.

<h4>Background</h4>Performing chloroplast DNA (cpDNA) isolation is considered a major challenge among different plant groups, especially conifers. Isolating chloroplasts in conifers by such conventional methods as sucrose gradient and high salt has not been successful. So far, plastid ge...

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Bibliographic Details
Main Authors: Leila do Nascimento Vieira, Helisson Faoro, Hugo Pacheco de Freitas Fraga, Marcelo Rogalski, Emanuel Maltempi de Souza, Fábio de Oliveira Pedrosa, Rubens Onofre Nodari, Miguel Pedro Guerra
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24392157/?tool=EBI

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24392157/?tool=EBI

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