Mechanical strain regulates osteoblast proliferation through integrin-mediated ERK activation.
Mechanical strain plays a critical role in the proliferation, differentiation and maturation of bone cells. As mechanical receptor cells, osteoblasts perceive and respond to stress force, such as those associated with compression, strain and shear stress. However, the underlying molecular mechanisms...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22539993/?tool=EBI |
| _version_ | 1818338137813811200 |
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| author | Yu-xian Yan Yuan-wei Gong Yong Guo Qi Lv Chun Guo Yan Zhuang Yuan Zhang Ruixin Li Xi-zheng Zhang |
| author_facet | Yu-xian Yan Yuan-wei Gong Yong Guo Qi Lv Chun Guo Yan Zhuang Yuan Zhang Ruixin Li Xi-zheng Zhang |
| author_sort | Yu-xian Yan |
| collection | DOAJ |
| description | Mechanical strain plays a critical role in the proliferation, differentiation and maturation of bone cells. As mechanical receptor cells, osteoblasts perceive and respond to stress force, such as those associated with compression, strain and shear stress. However, the underlying molecular mechanisms of this process remain unclear. Using a four-point bending device, mouse MC3T3-E1 cells was exposed to mechanical tensile strain. Cell proliferation was determined to be most efficient when stimulated once a day by mechanical strain at a frequency of 0.5 Hz and intensities of 2500 µε with once a day, and a periodicity of 1 h/day for 3 days. The applied mechanical strain resulted in the altered expression of 1992 genes, 41 of which are involved in the mitogen-activated protein kinase (MAPK) signaling pathway. Activation of ERK by mechanical strain promoted cell proliferation and inactivation of ERK by PD98059 suppressed proliferation, confirming that ERK plays an important role in the response to mechanical strain. Furthermore, the membrane-associated receptors integrin β1 and integrin β5 were determined to regulate ERK activity and the proliferation of mechanical strain-treated MC3T3-E1 cells in opposite ways. The knockdown of integrin β1 led to the inhibition of ERK activity and cell proliferation, whereas the knockdown of integrin β5 led to the enhancement of both processes. This study proposes a novel mechanism by which mechanical strain regulates bone growth and remodeling. |
| first_indexed | 2024-12-13T15:06:20Z |
| format | Article |
| id | doaj.art-e6bb730e82ea44008eeda1bfb565b212 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T15:06:20Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-e6bb730e82ea44008eeda1bfb565b2122022-12-21T23:40:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0174e3570910.1371/journal.pone.0035709Mechanical strain regulates osteoblast proliferation through integrin-mediated ERK activation.Yu-xian YanYuan-wei GongYong GuoQi LvChun GuoYan ZhuangYuan ZhangRuixin LiXi-zheng ZhangMechanical strain plays a critical role in the proliferation, differentiation and maturation of bone cells. As mechanical receptor cells, osteoblasts perceive and respond to stress force, such as those associated with compression, strain and shear stress. However, the underlying molecular mechanisms of this process remain unclear. Using a four-point bending device, mouse MC3T3-E1 cells was exposed to mechanical tensile strain. Cell proliferation was determined to be most efficient when stimulated once a day by mechanical strain at a frequency of 0.5 Hz and intensities of 2500 µε with once a day, and a periodicity of 1 h/day for 3 days. The applied mechanical strain resulted in the altered expression of 1992 genes, 41 of which are involved in the mitogen-activated protein kinase (MAPK) signaling pathway. Activation of ERK by mechanical strain promoted cell proliferation and inactivation of ERK by PD98059 suppressed proliferation, confirming that ERK plays an important role in the response to mechanical strain. Furthermore, the membrane-associated receptors integrin β1 and integrin β5 were determined to regulate ERK activity and the proliferation of mechanical strain-treated MC3T3-E1 cells in opposite ways. The knockdown of integrin β1 led to the inhibition of ERK activity and cell proliferation, whereas the knockdown of integrin β5 led to the enhancement of both processes. This study proposes a novel mechanism by which mechanical strain regulates bone growth and remodeling.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22539993/?tool=EBI |
| spellingShingle | Yu-xian Yan Yuan-wei Gong Yong Guo Qi Lv Chun Guo Yan Zhuang Yuan Zhang Ruixin Li Xi-zheng Zhang Mechanical strain regulates osteoblast proliferation through integrin-mediated ERK activation. PLoS ONE |
| title | Mechanical strain regulates osteoblast proliferation through integrin-mediated ERK activation. |
| title_full | Mechanical strain regulates osteoblast proliferation through integrin-mediated ERK activation. |
| title_fullStr | Mechanical strain regulates osteoblast proliferation through integrin-mediated ERK activation. |
| title_full_unstemmed | Mechanical strain regulates osteoblast proliferation through integrin-mediated ERK activation. |
| title_short | Mechanical strain regulates osteoblast proliferation through integrin-mediated ERK activation. |
| title_sort | mechanical strain regulates osteoblast proliferation through integrin mediated erk activation |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22539993/?tool=EBI |
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