Genetic Code Expansion and Click-Chemistry Labeling to Visualize GABA-A Receptors by Super-Resolution Microscopy

Genetic Code Expansion and Click-Chemistry Labeling to Visualize GABA-A Receptors by Super-Resolution Microscopy

Fluorescence labeling of difficult to access protein sites, e.g., in confined compartments, requires small fluorescent labels that can be covalently tethered at well-defined positions with high efficiency. Here, we report site-specific labeling of the extracellular domain of γ-aminobutyric acid type...

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Bibliographic Details
Main Authors: Alexander Kuhlemann, Gerti Beliu, Dieter Janzen, Enrica Maria Petrini, Danush Taban, Dominic A. Helmerich, Sören Doose, Martina Bruno, Andrea Barberis, Carmen Villmann, Markus Sauer, Christian Werner
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-11-01
Series:Frontiers in Synaptic Neuroscience
Subjects:
super-resolution microscopy (SRM)
click-chemistry
dSTORM
GABA-A receptor
genetic code expansion
Online Access:https://www.frontiersin.org/articles/10.3389/fnsyn.2021.727406/full

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https://www.frontiersin.org/articles/10.3389/fnsyn.2021.727406/full

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