HDX-MS: An Analytical Tool to Capture Protein Motion in Action
Virtually all protein functions in the cell, including pathogenic processes, require coordinated motion of atoms or domains, i.e., conformational dynamics. Understanding protein dynamics is therefore critical both for drug development and to learn about the underlying molecular causes of many diseas...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-07-01
|
| Series: | Biomedicines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9059/8/7/224 |
| _version_ | 1797562223158099968 |
|---|---|
| author | Dominic Narang Cristina Lento Derek J. Wilson |
| author_facet | Dominic Narang Cristina Lento Derek J. Wilson |
| author_sort | Dominic Narang |
| collection | DOAJ |
| description | Virtually all protein functions in the cell, including pathogenic processes, require coordinated motion of atoms or domains, i.e., conformational dynamics. Understanding protein dynamics is therefore critical both for drug development and to learn about the underlying molecular causes of many diseases. Hydrogen–Deuterium Exchange Mass Spectrometry (HDX-MS) provides valuable information about protein dynamics, which is highly complementary to the static picture provided by conventional high-resolution structural tools (i.e., X-ray crystallography and structural NMR). The amount of protein required to carry out HDX-MS experiments is a fraction of the amount required by alternative biophysical techniques, which are also usually lower resolution. Use of HDX-MS is growing quickly both in industry and academia, and it has been successfully used in numerous drug and vaccine development efforts, with important roles in understanding allosteric effects and mapping binding sites. |
| first_indexed | 2024-03-10T18:25:10Z |
| format | Article |
| id | doaj.art-e71d04450baf4781ad7f66c70915b97e |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T18:25:10Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-e71d04450baf4781ad7f66c70915b97e2023-11-20T07:07:08ZengMDPI AGBiomedicines2227-90592020-07-018722410.3390/biomedicines8070224HDX-MS: An Analytical Tool to Capture Protein Motion in ActionDominic Narang0Cristina Lento1Derek J. Wilson2Department of Chemistry, York University, Toronto, ON M3J 1P3, CanadaDepartment of Chemistry, York University, Toronto, ON M3J 1P3, CanadaDepartment of Chemistry, York University, Toronto, ON M3J 1P3, CanadaVirtually all protein functions in the cell, including pathogenic processes, require coordinated motion of atoms or domains, i.e., conformational dynamics. Understanding protein dynamics is therefore critical both for drug development and to learn about the underlying molecular causes of many diseases. Hydrogen–Deuterium Exchange Mass Spectrometry (HDX-MS) provides valuable information about protein dynamics, which is highly complementary to the static picture provided by conventional high-resolution structural tools (i.e., X-ray crystallography and structural NMR). The amount of protein required to carry out HDX-MS experiments is a fraction of the amount required by alternative biophysical techniques, which are also usually lower resolution. Use of HDX-MS is growing quickly both in industry and academia, and it has been successfully used in numerous drug and vaccine development efforts, with important roles in understanding allosteric effects and mapping binding sites.https://www.mdpi.com/2227-9059/8/7/224hydrogen–deuterium exchange mass spectrometryHDX-MSprotein dynamicsprotein folding and misfolding |
| spellingShingle | Dominic Narang Cristina Lento Derek J. Wilson HDX-MS: An Analytical Tool to Capture Protein Motion in Action Biomedicines hydrogen–deuterium exchange mass spectrometry HDX-MS protein dynamics protein folding and misfolding |
| title | HDX-MS: An Analytical Tool to Capture Protein Motion in Action |
| title_full | HDX-MS: An Analytical Tool to Capture Protein Motion in Action |
| title_fullStr | HDX-MS: An Analytical Tool to Capture Protein Motion in Action |
| title_full_unstemmed | HDX-MS: An Analytical Tool to Capture Protein Motion in Action |
| title_short | HDX-MS: An Analytical Tool to Capture Protein Motion in Action |
| title_sort | hdx ms an analytical tool to capture protein motion in action |
| topic | hydrogen–deuterium exchange mass spectrometry HDX-MS protein dynamics protein folding and misfolding |
| url | https://www.mdpi.com/2227-9059/8/7/224 |
| work_keys_str_mv | AT dominicnarang hdxmsananalyticaltooltocaptureproteinmotioninaction AT cristinalento hdxmsananalyticaltooltocaptureproteinmotioninaction AT derekjwilson hdxmsananalyticaltooltocaptureproteinmotioninaction |