Network pharmacology and experimental verification reveal the mechanism of safranal against glioblastoma (GBM)
IntroductionSafranal is an active component of the traditional Tibetan medicine (TTM) saffron, which has potential anticancer activity.Methods and resultsHere, we studied the therapeutic effect and mechanism of safranal on GBM. CCK-8, GBM-brain organoid coculture experiments and 3D tumour spheroid i...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2023.1255164/full |
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| author | Xiaobing Yang Xiaobing Yang Di Lu Di Lu Yanfei Sun Yanfei Sun Tiandi Wei Dulegeqi Man Anbin Chen Tao Luo Tao Luo Feihu Zhao Feihu Zhao Xuemeng Liu Xuemeng Liu Bo Cheng Bo Cheng Xu Wang Xu Wang Peng Zhao Peng Zhao Donghai Wang Donghai Wang Xingang Li Xingang Li |
| author_facet | Xiaobing Yang Xiaobing Yang Di Lu Di Lu Yanfei Sun Yanfei Sun Tiandi Wei Dulegeqi Man Anbin Chen Tao Luo Tao Luo Feihu Zhao Feihu Zhao Xuemeng Liu Xuemeng Liu Bo Cheng Bo Cheng Xu Wang Xu Wang Peng Zhao Peng Zhao Donghai Wang Donghai Wang Xingang Li Xingang Li |
| author_sort | Xiaobing Yang |
| collection | DOAJ |
| description | IntroductionSafranal is an active component of the traditional Tibetan medicine (TTM) saffron, which has potential anticancer activity.Methods and resultsHere, we studied the therapeutic effect and mechanism of safranal on GBM. CCK-8, GBM-brain organoid coculture experiments and 3D tumour spheroid invasion assays showed that safranal inhibited GBM cell proliferation and invasion in vitro. Network pharmacology, RNA-seq, molecular docking analysis, western blotting, apoptosis, and cell cycle assays predicted and verified that safranal could promote GBM cell apoptosis and G2/M phase arrest and inhibit the PI3K/AKT/mTOR axis. In vivo experiments showed that safranal could inhibit GBM cell growth alone and in combination with TMZ.ConclusionThis study revealed that safranal inhibits GBM cell growth in vivo and in vitro, promotes GBM cell apoptosis and G2/M phase arrest, inhibits the PI3K/AKT/mTOR axis and cooperate with TMZ. |
| first_indexed | 2024-03-12T02:26:29Z |
| format | Article |
| id | doaj.art-e73fbf72d5ae40219aa6e29e604cb646 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-03-12T02:26:29Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-e73fbf72d5ae40219aa6e29e604cb6462023-09-05T12:30:46ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2023-09-011310.3389/fonc.2023.12551641255164Network pharmacology and experimental verification reveal the mechanism of safranal against glioblastoma (GBM)Xiaobing Yang0Xiaobing Yang1Di Lu2Di Lu3Yanfei Sun4Yanfei Sun5Tiandi Wei6Dulegeqi Man7Anbin Chen8Tao Luo9Tao Luo10Feihu Zhao11Feihu Zhao12Xuemeng Liu13Xuemeng Liu14Bo Cheng15Bo Cheng16Xu Wang17Xu Wang18Peng Zhao19Peng Zhao20Donghai Wang21Donghai Wang22Xingang Li23Xingang Li24Department of Neurosurgery, Qilu Hospital, Cheeloo College of Medicine and Institute of Brain and Brain-Inspired Science, Shandong University, Jinan, ChinaJinan Microecological Biomedicine Shandong Laboratory and Shandong Key Laboratory of Brain Function Remodeling, Jinan, ChinaDepartment of Neurosurgery, Qilu Hospital, Cheeloo College of Medicine and Institute of Brain and Brain-Inspired Science, Shandong University, Jinan, ChinaJinan Microecological Biomedicine Shandong Laboratory and Shandong Key Laboratory of Brain Function Remodeling, Jinan, ChinaDepartment of Neurosurgery, Qilu Hospital, Cheeloo College of Medicine and Institute of Brain and Brain-Inspired Science, Shandong University, Jinan, ChinaJinan Microecological Biomedicine Shandong Laboratory and Shandong Key Laboratory of Brain Function Remodeling, Jinan, ChinaState Key Laboratory of Microbial Technology, Microbial Technology, Institute, Shandong University, Qingdao, ChinaInternational Mongolia Hospital of Inner Mongolia, Hohhot, ChinaDepartment of Neurosurgery, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, ChinaSchool of Basic Medical Sciences, Guizhou Medical University, Guiyang, ChinaDepartment of Emergency, The Affiliated Hospital of Guizhou Medical University, Guizhou Medical University, Guiyang, ChinaDepartment of Neurosurgery, Qilu Hospital, Cheeloo College of Medicine and Institute of Brain and Brain-Inspired Science, Shandong University, Jinan, ChinaJinan Microecological Biomedicine Shandong Laboratory and Shandong Key Laboratory of Brain Function Remodeling, Jinan, ChinaDepartment of Neurosurgery, Qilu Hospital, Cheeloo College of Medicine and Institute of Brain and Brain-Inspired Science, Shandong University, Jinan, ChinaJinan Microecological Biomedicine Shandong Laboratory and Shandong Key Laboratory of Brain Function Remodeling, Jinan, ChinaDepartment of Neurosurgery, Qilu Hospital, Cheeloo College of Medicine and Institute of Brain and Brain-Inspired Science, Shandong University, Jinan, ChinaJinan Microecological Biomedicine Shandong Laboratory and Shandong Key Laboratory of Brain Function Remodeling, Jinan, ChinaDepartment of Neurosurgery, Qilu Hospital, Cheeloo College of Medicine and Institute of Brain and Brain-Inspired Science, Shandong University, Jinan, ChinaJinan Microecological Biomedicine Shandong Laboratory and Shandong Key Laboratory of Brain Function Remodeling, Jinan, ChinaDepartment of Neurosurgery, Qilu Hospital, Cheeloo College of Medicine and Institute of Brain and Brain-Inspired Science, Shandong University, Jinan, ChinaJinan Microecological Biomedicine Shandong Laboratory and Shandong Key Laboratory of Brain Function Remodeling, Jinan, ChinaDepartment of Neurosurgery, Qilu Hospital, Cheeloo College of Medicine and Institute of Brain and Brain-Inspired Science, Shandong University, Jinan, ChinaJinan Microecological Biomedicine Shandong Laboratory and Shandong Key Laboratory of Brain Function Remodeling, Jinan, ChinaDepartment of Neurosurgery, Qilu Hospital, Cheeloo College of Medicine and Institute of Brain and Brain-Inspired Science, Shandong University, Jinan, ChinaJinan Microecological Biomedicine Shandong Laboratory and Shandong Key Laboratory of Brain Function Remodeling, Jinan, ChinaIntroductionSafranal is an active component of the traditional Tibetan medicine (TTM) saffron, which has potential anticancer activity.Methods and resultsHere, we studied the therapeutic effect and mechanism of safranal on GBM. CCK-8, GBM-brain organoid coculture experiments and 3D tumour spheroid invasion assays showed that safranal inhibited GBM cell proliferation and invasion in vitro. Network pharmacology, RNA-seq, molecular docking analysis, western blotting, apoptosis, and cell cycle assays predicted and verified that safranal could promote GBM cell apoptosis and G2/M phase arrest and inhibit the PI3K/AKT/mTOR axis. In vivo experiments showed that safranal could inhibit GBM cell growth alone and in combination with TMZ.ConclusionThis study revealed that safranal inhibits GBM cell growth in vivo and in vitro, promotes GBM cell apoptosis and G2/M phase arrest, inhibits the PI3K/AKT/mTOR axis and cooperate with TMZ.https://www.frontiersin.org/articles/10.3389/fonc.2023.1255164/fullgliomatraditional tibetan medicinesafranalAktnetwork pharmacology |
| spellingShingle | Xiaobing Yang Xiaobing Yang Di Lu Di Lu Yanfei Sun Yanfei Sun Tiandi Wei Dulegeqi Man Anbin Chen Tao Luo Tao Luo Feihu Zhao Feihu Zhao Xuemeng Liu Xuemeng Liu Bo Cheng Bo Cheng Xu Wang Xu Wang Peng Zhao Peng Zhao Donghai Wang Donghai Wang Xingang Li Xingang Li Network pharmacology and experimental verification reveal the mechanism of safranal against glioblastoma (GBM) Frontiers in Oncology glioma traditional tibetan medicine safranal Akt network pharmacology |
| title | Network pharmacology and experimental verification reveal the mechanism of safranal against glioblastoma (GBM) |
| title_full | Network pharmacology and experimental verification reveal the mechanism of safranal against glioblastoma (GBM) |
| title_fullStr | Network pharmacology and experimental verification reveal the mechanism of safranal against glioblastoma (GBM) |
| title_full_unstemmed | Network pharmacology and experimental verification reveal the mechanism of safranal against glioblastoma (GBM) |
| title_short | Network pharmacology and experimental verification reveal the mechanism of safranal against glioblastoma (GBM) |
| title_sort | network pharmacology and experimental verification reveal the mechanism of safranal against glioblastoma gbm |
| topic | glioma traditional tibetan medicine safranal Akt network pharmacology |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2023.1255164/full |
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