Video-based calcification assay: A novel method for kinetic analysis of osteogenesis in live cultures

Traditional methods of quantifying osteoblast calcification in culture require the use of calcium sensitive dyes, such as Arsenazo III or Alizarin Red S, which have been successfully used for decades to assess osteogenesis. Because these dyes elicit a colorimetric change when reacted with a cell lys...

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Bibliographic Details
Main Authors: Ivann KC Martinez, Bir Bhanu, Nicole I zur Nieden
Format: Article
Language:English
Published: Elsevier 2021-01-01
Series:MethodsX
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016121000583