Video-based calcification assay: A novel method for kinetic analysis of osteogenesis in live cultures
Traditional methods of quantifying osteoblast calcification in culture require the use of calcium sensitive dyes, such as Arsenazo III or Alizarin Red S, which have been successfully used for decades to assess osteogenesis. Because these dyes elicit a colorimetric change when reacted with a cell lys...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2021-01-01
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Series: | MethodsX |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016121000583 |