DNA and RNA Extraction and Quantitative Real-Time PCR-Based Assays for Biogas Biocenoses in an Interlaboratory Comparison

DNA and RNA Extraction and Quantitative Real-Time PCR-Based Assays for Biogas Biocenoses in an Interlaboratory Comparison

Five institutional partners participated in an interlaboratory comparison of nucleic acid extraction, RNA preservation and quantitative Real-Time PCR (qPCR) based assays for biogas biocenoses derived from different grass silage digesting laboratory and pilot scale fermenters. A kit format DNA extrac...

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Bibliographic Details
Main Authors: Michael Lebuhn, Jaqueline Derenkó, Antje Rademacher, Susanne Helbig, Bernhard Munk, Alexander Pechtl, Yvonne Stolze, Steffen Prowe, Wolfgang H. Schwarz, Andreas Schlüter, Wolfgang Liebl, Michael Klocke
Format: Article
Language:English
Published: MDPI AG 2016-01-01
Series:Bioengineering
Subjects:
ring-trial
fermenter sludge
DNA purity
PCR suitability
extraction efficiency
RNA preservation
reverse transcription
methanogens
Bacteria
absolute quantification
Online Access:http://www.mdpi.com/2306-5354/3/1/7

Internet

http://www.mdpi.com/2306-5354/3/1/7

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