An Allosteric-Probe for Detection of Alkaline Phosphatase Activity and Its Application in Immunoassay

An Allosteric-Probe for Detection of Alkaline Phosphatase Activity and Its Application in Immunoassay

A fluorescence strategy for alkaline phosphatase (ALP) assay in complicated samples with high sensitivity and strong stability is developed based on an allosteric probe (AP). This probe consists of two DNA strands, a streptavidin (SA) aptamer labeled by fluorophore and its totally complementary DNA...

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Bibliographic Details
Main Authors: Jingjing Guo, Mingxuan Gao, Yanling Song, Li Lin, Kaifeng Zhao, Tian Tian, Dan Liu, Zhi Zhu, Chaoyong James Yang
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-12-01
Series:Frontiers in Chemistry
Subjects:
aptamer
alkaline phosphatase
allosteric-probes
complex sample
immunoassay
Online Access:https://www.frontiersin.org/article/10.3389/fchem.2018.00618/full

Internet

https://www.frontiersin.org/article/10.3389/fchem.2018.00618/full

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