Short‐chain fatty acids as a target for prevention against food allergy by regulatory T cells
Objective Food allergy (FA) has become a public health issue of global concern. Short‐chain fatty acids (SCFAs) are one of the most important biomarkers of intestinal metabolites. SCFAs may affect the occurrence and development of FA. Currently, no studies have been reported on the mechanism of FA i...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-06-01
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| Series: | JGH Open |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/jgh3.12130 |
| _version_ | 1818461942527819776 |
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| author | Zhenni Zhu Bin Zhu Chijun Hu Yang Liu Xiaoli Wang Jiajia Zhang Fengge Wang Maolong Zhu |
| author_facet | Zhenni Zhu Bin Zhu Chijun Hu Yang Liu Xiaoli Wang Jiajia Zhang Fengge Wang Maolong Zhu |
| author_sort | Zhenni Zhu |
| collection | DOAJ |
| description | Objective Food allergy (FA) has become a public health issue of global concern. Short‐chain fatty acids (SCFAs) are one of the most important biomarkers of intestinal metabolites. SCFAs may affect the occurrence and development of FA. Currently, no studies have been reported on the mechanism of FA in response to SCFAs. In this study, the common food allergen ovalbumin (OVA) was used for intestinal sensitization in Balb/c mice to study the effect of FA on intestinal barrier function and regulatory T cells in mice, thus providing a new target for the prevention and treatment of FA. Methods Twenty BALB/c mice were randomly divided into the experimental group and control group. The experimental group was given OVA, and the control group was given an equal amount of physiological saline. On the 31st day of modeling, the levels of secretory immunoglobulin A (sIgA) and serum total IgE and diamine oxidase (DAO) were determined using enzyme linked immunosorbent assay (ELISA). At the same time, after in vitro stimulation with different concentrations of SCFAs and histone acetylase inhibitor trichostatin A (TSA), the frequency and function of Treg in OVA‐sensitized mice were detected by flow cytometry. Results Different concentrations of SCFAs and TSA selectively proliferate Treg cells in a dose‐dependent manner. SCFAs and TSA‐pretreated PBMCs that were injected intravenously into the OVA‐sensitized mice through the tail vein can significantly reduce the expression of IgE, DAO, and sIgA. Conclusion SCFAs and TSA can selectively proliferate Tregs and upregulate the expression of anti‐inflammatory cytokines, thereby suppressing allergic reactions. |
| first_indexed | 2024-12-14T23:54:09Z |
| format | Article |
| id | doaj.art-e76c2e33f4a644c3ba7acb9c59ac9bda |
| institution | Directory Open Access Journal |
| issn | 2397-9070 |
| language | English |
| last_indexed | 2024-12-14T23:54:09Z |
| publishDate | 2019-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | JGH Open |
| spelling | doaj.art-e76c2e33f4a644c3ba7acb9c59ac9bda2022-12-21T22:43:10ZengWileyJGH Open2397-90702019-06-013319019510.1002/jgh3.12130Short‐chain fatty acids as a target for prevention against food allergy by regulatory T cellsZhenni Zhu0Bin Zhu1Chijun Hu2Yang Liu3Xiaoli Wang4Jiajia Zhang5Fengge Wang6Maolong Zhu7Department of Child Gastroenterology Maternal and Child Health Hospital of Hubei Province (Women and Children's Hospital of Hubei Province) Wuhan Hubei ChinaDepartment of Infectious Disease, Union Hospital, Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei ChinaDepartment of Child Gastroenterology Maternal and Child Health Hospital of Hubei Province (Women and Children's Hospital of Hubei Province) Wuhan Hubei ChinaDepartment of Child Gastroenterology Maternal and Child Health Hospital of Hubei Province (Women and Children's Hospital of Hubei Province) Wuhan Hubei ChinaDepartment of Child Gastroenterology Maternal and Child Health Hospital of Hubei Province (Women and Children's Hospital of Hubei Province) Wuhan Hubei ChinaDepartment of Child Gastroenterology Maternal and Child Health Hospital of Hubei Province (Women and Children's Hospital of Hubei Province) Wuhan Hubei ChinaDepartment of Child Gastroenterology Maternal and Child Health Hospital of Hubei Province (Women and Children's Hospital of Hubei Province) Wuhan Hubei ChinaDepartment of Child Gastroenterology Maternal and Child Health Hospital of Hubei Province (Women and Children's Hospital of Hubei Province) Wuhan Hubei ChinaObjective Food allergy (FA) has become a public health issue of global concern. Short‐chain fatty acids (SCFAs) are one of the most important biomarkers of intestinal metabolites. SCFAs may affect the occurrence and development of FA. Currently, no studies have been reported on the mechanism of FA in response to SCFAs. In this study, the common food allergen ovalbumin (OVA) was used for intestinal sensitization in Balb/c mice to study the effect of FA on intestinal barrier function and regulatory T cells in mice, thus providing a new target for the prevention and treatment of FA. Methods Twenty BALB/c mice were randomly divided into the experimental group and control group. The experimental group was given OVA, and the control group was given an equal amount of physiological saline. On the 31st day of modeling, the levels of secretory immunoglobulin A (sIgA) and serum total IgE and diamine oxidase (DAO) were determined using enzyme linked immunosorbent assay (ELISA). At the same time, after in vitro stimulation with different concentrations of SCFAs and histone acetylase inhibitor trichostatin A (TSA), the frequency and function of Treg in OVA‐sensitized mice were detected by flow cytometry. Results Different concentrations of SCFAs and TSA selectively proliferate Treg cells in a dose‐dependent manner. SCFAs and TSA‐pretreated PBMCs that were injected intravenously into the OVA‐sensitized mice through the tail vein can significantly reduce the expression of IgE, DAO, and sIgA. Conclusion SCFAs and TSA can selectively proliferate Tregs and upregulate the expression of anti‐inflammatory cytokines, thereby suppressing allergic reactions.https://doi.org/10.1002/jgh3.12130food allergyregulatory T cellsshort‐chain fatty acids |
| spellingShingle | Zhenni Zhu Bin Zhu Chijun Hu Yang Liu Xiaoli Wang Jiajia Zhang Fengge Wang Maolong Zhu Short‐chain fatty acids as a target for prevention against food allergy by regulatory T cells JGH Open food allergy regulatory T cells short‐chain fatty acids |
| title | Short‐chain fatty acids as a target for prevention against food allergy by regulatory T cells |
| title_full | Short‐chain fatty acids as a target for prevention against food allergy by regulatory T cells |
| title_fullStr | Short‐chain fatty acids as a target for prevention against food allergy by regulatory T cells |
| title_full_unstemmed | Short‐chain fatty acids as a target for prevention against food allergy by regulatory T cells |
| title_short | Short‐chain fatty acids as a target for prevention against food allergy by regulatory T cells |
| title_sort | short chain fatty acids as a target for prevention against food allergy by regulatory t cells |
| topic | food allergy regulatory T cells short‐chain fatty acids |
| url | https://doi.org/10.1002/jgh3.12130 |
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