Optimized protocol to derive germline stem-cell-like cells from mouse pluripotent stem cells

Optimized protocol to derive germline stem-cell-like cells from mouse pluripotent stem cells

Summary: Male germ-cell development comprises primordial germ-cell (PGC) development, spermatogonium differentiation, and ensuing spermatogenesis. We present a step-by-step protocol for differentiation of mouse pluripotent stem cells (PSCs) into germline stem-cell-like cells (GSCLCs) via PGC-like ce...

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Bibliographic Details
Main Authors: Yukiko Ishikura, Hiroshi Ohta, Masahiro Nagano, Mitinori Saitou
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:STAR Protocols
Subjects:
Developmental biology
Stem Cells
Cell Differentiation
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722004245

Internet

http://www.sciencedirect.com/science/article/pii/S2666166722004245

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