Kinetics of LYVE-1-positive M2-like macrophages in developing and repairing dental pulp in vivo and their pro-angiogenic activity in vitro
Abstract Tissue-resident macrophages expressing lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) are found in multiple tissues and organs. We aimed to evaluate the dynamics and biological functions of LYVE-1+ macrophages in dental pulp during post-injury tissue remodeling. Immunofluoresce...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-08987-3 |
| _version_ | 1818776722709938176 |
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| author | Thoai Quoc Kieu Kento Tazawa Nobuyuki Kawashima Sonoko Noda Mayuko Fujii Keisuke Nara Kentaro Hashimoto Peifeng Han Takashi Okiji |
| author_facet | Thoai Quoc Kieu Kento Tazawa Nobuyuki Kawashima Sonoko Noda Mayuko Fujii Keisuke Nara Kentaro Hashimoto Peifeng Han Takashi Okiji |
| author_sort | Thoai Quoc Kieu |
| collection | DOAJ |
| description | Abstract Tissue-resident macrophages expressing lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) are found in multiple tissues and organs. We aimed to evaluate the dynamics and biological functions of LYVE-1+ macrophages in dental pulp during post-injury tissue remodeling. Immunofluorescence staining of mouse embryos revealed that LYVE-1+ macrophages colonized dental pulp before birth. In mature rat molar dental pulp, LYVE-1+ macrophages were the main subset of macrophages expressing CD163, an M2 marker, and were distributed throughout the tissue. In response to dental pulp injury induced by cavity preparation, LYVE-1+ macrophages quickly disappeared from the affected area of the pulp and gradually repopulated during the wound healing process. RAW264.7 mouse macrophages cultured with a mixture of macrophage colony-stimulating factor, interleukin-4, and dexamethasone increased LYVE-1 expression, whereas lipopolysaccharide-stimulation decreased LYVE-1 expression. Enforced expression of Lyve1 in RAW264.7 cells resulted in increased mRNA expression of matrix metalloproteinase 2 (Mmp2), Mmp9, and vascular endothelial growth factor A (Vegfa). Lyve1-expressing macrophages promoted the migration and tube formation of human umbilical vein endothelial cells. In conclusion, LYVE-1+ tissue-resident M2-like macrophages in dental pulp showed dynamism in response to pulp injury, and possibly play an important role in angiogenesis during wound healing and tissue remodeling. |
| first_indexed | 2024-12-18T11:17:27Z |
| format | Article |
| id | doaj.art-e785b702e5b54909a4293f239fa80d98 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-18T11:17:27Z |
| publishDate | 2022-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-e785b702e5b54909a4293f239fa80d982022-12-21T21:09:55ZengNature PortfolioScientific Reports2045-23222022-03-0112111410.1038/s41598-022-08987-3Kinetics of LYVE-1-positive M2-like macrophages in developing and repairing dental pulp in vivo and their pro-angiogenic activity in vitroThoai Quoc Kieu0Kento Tazawa1Nobuyuki Kawashima2Sonoko Noda3Mayuko Fujii4Keisuke Nara5Kentaro Hashimoto6Peifeng Han7Takashi Okiji8Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU)Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU)Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU)Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU)Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU)Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU)Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU)Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU)Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU)Abstract Tissue-resident macrophages expressing lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) are found in multiple tissues and organs. We aimed to evaluate the dynamics and biological functions of LYVE-1+ macrophages in dental pulp during post-injury tissue remodeling. Immunofluorescence staining of mouse embryos revealed that LYVE-1+ macrophages colonized dental pulp before birth. In mature rat molar dental pulp, LYVE-1+ macrophages were the main subset of macrophages expressing CD163, an M2 marker, and were distributed throughout the tissue. In response to dental pulp injury induced by cavity preparation, LYVE-1+ macrophages quickly disappeared from the affected area of the pulp and gradually repopulated during the wound healing process. RAW264.7 mouse macrophages cultured with a mixture of macrophage colony-stimulating factor, interleukin-4, and dexamethasone increased LYVE-1 expression, whereas lipopolysaccharide-stimulation decreased LYVE-1 expression. Enforced expression of Lyve1 in RAW264.7 cells resulted in increased mRNA expression of matrix metalloproteinase 2 (Mmp2), Mmp9, and vascular endothelial growth factor A (Vegfa). Lyve1-expressing macrophages promoted the migration and tube formation of human umbilical vein endothelial cells. In conclusion, LYVE-1+ tissue-resident M2-like macrophages in dental pulp showed dynamism in response to pulp injury, and possibly play an important role in angiogenesis during wound healing and tissue remodeling.https://doi.org/10.1038/s41598-022-08987-3 |
| spellingShingle | Thoai Quoc Kieu Kento Tazawa Nobuyuki Kawashima Sonoko Noda Mayuko Fujii Keisuke Nara Kentaro Hashimoto Peifeng Han Takashi Okiji Kinetics of LYVE-1-positive M2-like macrophages in developing and repairing dental pulp in vivo and their pro-angiogenic activity in vitro Scientific Reports |
| title | Kinetics of LYVE-1-positive M2-like macrophages in developing and repairing dental pulp in vivo and their pro-angiogenic activity in vitro |
| title_full | Kinetics of LYVE-1-positive M2-like macrophages in developing and repairing dental pulp in vivo and their pro-angiogenic activity in vitro |
| title_fullStr | Kinetics of LYVE-1-positive M2-like macrophages in developing and repairing dental pulp in vivo and their pro-angiogenic activity in vitro |
| title_full_unstemmed | Kinetics of LYVE-1-positive M2-like macrophages in developing and repairing dental pulp in vivo and their pro-angiogenic activity in vitro |
| title_short | Kinetics of LYVE-1-positive M2-like macrophages in developing and repairing dental pulp in vivo and their pro-angiogenic activity in vitro |
| title_sort | kinetics of lyve 1 positive m2 like macrophages in developing and repairing dental pulp in vivo and their pro angiogenic activity in vitro |
| url | https://doi.org/10.1038/s41598-022-08987-3 |
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