Scalable, cGMP-compatible purification of extracellular vesicles carrying bioactive human heterodimeric IL-15/lactadherin complexes

Scalable, cGMP-compatible purification of extracellular vesicles carrying bioactive human heterodimeric IL-15/lactadherin complexes

The development of extracellular vesicles (EV) for therapeutic applications is contingent upon the establishment of reproducible, scalable, and high-throughput methods for the production and purification of clinical grade EV. Methods including ultracentrifugation (U/C), ultrafiltration, immunoprecip...

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Bibliographic Details
Main Authors: Dionysios C. Watson, Bryant C. Yung, Cristina Bergamaschi, Bhabadeb Chowdhury, Jenifer Bear, Dimitris Stellas, Aizea Morales-Kastresana, Jennifer C. Jones, Barbara K. Felber, Xiaoyuan Chen, George N. Pavlakis
Format: Article
Language:English
Published: Wiley 2018-12-01
Series:Journal of Extracellular Vesicles
Subjects:
Exosomes
clinical grade
immunotherapy
bioreactor
purification
large scale
tangential flow filtration
size-exclusion chromatography
interleukin-15
lactadherin
Online Access:http://dx.doi.org/10.1080/20013078.2018.1442088

Internet

http://dx.doi.org/10.1080/20013078.2018.1442088

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