Beta-glucan protects against isoproterenol-induced cardiac remodeling by regulating the ACE-AT1R axis and attenuates cardiac inflammation and apoptosis
Objective: To investigate the cardioprotective effect of beta-glucan against isoproterenol-induced cardiotoxicity in rats, and elucidate the underlying mechanism. Methods: Rats were orally pretreated with beta-glucan (40 mg/kg body weight) for 30 d, and isoproterenol (20 mg/100 g body weight) was ad...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2023-01-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.apjtb.org/article.asp?issn=2221-1691;year=2023;volume=13;issue=9;spage=384;epage=392;aulast=Roy |
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| author | Anitha Roy Vasantha Mallenahalli Neelakantappa Jayashree Ganesan Balakrishnan Ramajayam Asokan Srinivasan Kulandaivel V V Sathibabu Uddandrao Sengottuvelu Singaravel |
| author_facet | Anitha Roy Vasantha Mallenahalli Neelakantappa Jayashree Ganesan Balakrishnan Ramajayam Asokan Srinivasan Kulandaivel V V Sathibabu Uddandrao Sengottuvelu Singaravel |
| author_sort | Anitha Roy |
| collection | DOAJ |
| description | Objective: To investigate the cardioprotective effect of beta-glucan against isoproterenol-induced cardiotoxicity in rats, and elucidate the underlying mechanism.
Methods: Rats were orally pretreated with beta-glucan (40 mg/kg body weight) for 30 d, and isoproterenol (20 mg/100 g body weight) was administered on days 31 and 32. The effects of beta-glucan on markers of cardiac injury, hemodynamic changes, production of proinflammatory cytokines, and the corresponding mRNA expressions were evaluated. In addition, histological analysis was performed.
Results: Pretreatment with beta-glucan prevented isoproterenol-induced cardiac injury by preserving the structural and functional integrity of the plasma membrane and attenuating the production of proinflammatory cytokines (NF-κB, TNF-α, IL-6, IL-Ιβ, and IFN-γ) in the heart. Moreover, beta-glucan significantly downregulated the mRNA expression of ACE, AT1R, TNF-α, IL-6, NF-κB, caspase-3, TLR-4, and Bax, and upregulated Bcl-2 in the heart. At the same time, pretreatment with beta-glucan alleviated myocardial damage as reflected in a reduction in myonecrosis, edema, and erythrocyte extravasation with almost imperceptible inflammation.
Conclusions: Beta-glucan can protect against isoproterenol-induced cardiotoxicity by attenuating cardiac inflammation and apoptosis and regulating the ACE-AT1R axis, thereby preventing cardiac remodeling. |
| first_indexed | 2024-03-11T15:49:27Z |
| format | Article |
| id | doaj.art-e78fea4f59724dcc90dad908bed95704 |
| institution | Directory Open Access Journal |
| issn | 2221-1691 2588-9222 |
| language | English |
| last_indexed | 2024-03-11T15:49:27Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-e78fea4f59724dcc90dad908bed957042023-10-26T05:33:09ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912588-92222023-01-0113938439210.4103/2221-1691.385569Beta-glucan protects against isoproterenol-induced cardiac remodeling by regulating the ACE-AT1R axis and attenuates cardiac inflammation and apoptosisAnitha RoyVasantha Mallenahalli NeelakantappaJayashree GanesanBalakrishnan Ramajayam AsokanSrinivasan KulandaivelV V Sathibabu UddandraoSengottuvelu SingaravelObjective: To investigate the cardioprotective effect of beta-glucan against isoproterenol-induced cardiotoxicity in rats, and elucidate the underlying mechanism. Methods: Rats were orally pretreated with beta-glucan (40 mg/kg body weight) for 30 d, and isoproterenol (20 mg/100 g body weight) was administered on days 31 and 32. The effects of beta-glucan on markers of cardiac injury, hemodynamic changes, production of proinflammatory cytokines, and the corresponding mRNA expressions were evaluated. In addition, histological analysis was performed. Results: Pretreatment with beta-glucan prevented isoproterenol-induced cardiac injury by preserving the structural and functional integrity of the plasma membrane and attenuating the production of proinflammatory cytokines (NF-κB, TNF-α, IL-6, IL-Ιβ, and IFN-γ) in the heart. Moreover, beta-glucan significantly downregulated the mRNA expression of ACE, AT1R, TNF-α, IL-6, NF-κB, caspase-3, TLR-4, and Bax, and upregulated Bcl-2 in the heart. At the same time, pretreatment with beta-glucan alleviated myocardial damage as reflected in a reduction in myonecrosis, edema, and erythrocyte extravasation with almost imperceptible inflammation. Conclusions: Beta-glucan can protect against isoproterenol-induced cardiotoxicity by attenuating cardiac inflammation and apoptosis and regulating the ACE-AT1R axis, thereby preventing cardiac remodeling.http://www.apjtb.org/article.asp?issn=2221-1691;year=2023;volume=13;issue=9;spage=384;epage=392;aulast=Royβeta-glucan; isoproterenol; cardiac inflammation; cardiac apoptosis; cardiovascular diseases; heart failure; myocardial infarction |
| spellingShingle | Anitha Roy Vasantha Mallenahalli Neelakantappa Jayashree Ganesan Balakrishnan Ramajayam Asokan Srinivasan Kulandaivel V V Sathibabu Uddandrao Sengottuvelu Singaravel Beta-glucan protects against isoproterenol-induced cardiac remodeling by regulating the ACE-AT1R axis and attenuates cardiac inflammation and apoptosis Asian Pacific Journal of Tropical Biomedicine βeta-glucan; isoproterenol; cardiac inflammation; cardiac apoptosis; cardiovascular diseases; heart failure; myocardial infarction |
| title | Beta-glucan protects against isoproterenol-induced cardiac remodeling by regulating the ACE-AT1R axis and attenuates cardiac inflammation and apoptosis |
| title_full | Beta-glucan protects against isoproterenol-induced cardiac remodeling by regulating the ACE-AT1R axis and attenuates cardiac inflammation and apoptosis |
| title_fullStr | Beta-glucan protects against isoproterenol-induced cardiac remodeling by regulating the ACE-AT1R axis and attenuates cardiac inflammation and apoptosis |
| title_full_unstemmed | Beta-glucan protects against isoproterenol-induced cardiac remodeling by regulating the ACE-AT1R axis and attenuates cardiac inflammation and apoptosis |
| title_short | Beta-glucan protects against isoproterenol-induced cardiac remodeling by regulating the ACE-AT1R axis and attenuates cardiac inflammation and apoptosis |
| title_sort | beta glucan protects against isoproterenol induced cardiac remodeling by regulating the ace at1r axis and attenuates cardiac inflammation and apoptosis |
| topic | βeta-glucan; isoproterenol; cardiac inflammation; cardiac apoptosis; cardiovascular diseases; heart failure; myocardial infarction |
| url | http://www.apjtb.org/article.asp?issn=2221-1691;year=2023;volume=13;issue=9;spage=384;epage=392;aulast=Roy |
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