Long oligodeoxynucleotides: chemical synthesis, isolation via catching-by-polymerization, verification via sequencing, and gene expression demonstration

Long oligodeoxynucleotides: chemical synthesis, isolation via catching-by-polymerization, verification via sequencing, and gene expression demonstration

Long oligodeoxynucleotides (ODNs) are segments of DNAs having over one hundred nucleotides (nt). They are typically assembled using enzymatic methods such as PCR and ligation from shorter 20 to 60 nt ODNs produced by automated de novo chemical synthesis. While these methods have made many projects i...

Full description

Bibliographic Details
Main Authors: Yipeng Yin, Reed Arneson, Alexander Apostle, Adikari M. D. N. Eriyagama, Komal Chillar, Emma Burke, Martina Jahfetson, Yinan Yuan, Shiyue Fang
Format: Article
Language:English
Published: Beilstein-Institut 2023-12-01
Series:Beilstein Journal of Organic Chemistry
Subjects:
automated synthesis
catching-by-polymerization
gene assembly
long oligonucleotide
synthetic biology
Online Access:https://doi.org/10.3762/bjoc.19.146

Internet

https://doi.org/10.3762/bjoc.19.146

Similar Items