Effect of the consumption of brazzein and monellin, two recombinant sweet-tasting proteins, on rat gut microbiota
Sweet-tasting proteins (SPs) are proteins of plant origin initially isolated from tropical fruits. They are thousands of times sweeter than sucrose and most artificial sweeteners. SPs are a class of proteins capable of causing a sweet taste sensation in humans when interacting with the T1R2/T1R3 rec...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2024.1362529/full |
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| author | Vladimir A. Veselovsky Daria I. Boldyreva Evgenii I. Olekhnovich Ksenia M. Klimina Vladislav V. Babenko Natalia V. Zakharevich Andrey K. Larin Maxim D. Morozov Polina Y. Zoruk Petr V. Sergiev Olga A. Dontsova Igor V. Maev Tamara S. Novik Anatoly A. Kotlobay Vassili N. Lazarev Maria A. Lagarkova |
| author_facet | Vladimir A. Veselovsky Daria I. Boldyreva Evgenii I. Olekhnovich Ksenia M. Klimina Vladislav V. Babenko Natalia V. Zakharevich Andrey K. Larin Maxim D. Morozov Polina Y. Zoruk Petr V. Sergiev Olga A. Dontsova Igor V. Maev Tamara S. Novik Anatoly A. Kotlobay Vassili N. Lazarev Maria A. Lagarkova |
| author_sort | Vladimir A. Veselovsky |
| collection | DOAJ |
| description | Sweet-tasting proteins (SPs) are proteins of plant origin initially isolated from tropical fruits. They are thousands of times sweeter than sucrose and most artificial sweeteners. SPs are a class of proteins capable of causing a sweet taste sensation in humans when interacting with the T1R2/T1R3 receptor. SP thaumatin has already been introduced in the food industry in some countries. Other SPs, such as monellin and brazzein, are promising products. An important stage in researching SPs, in addition to confirming the absence of toxicity, mutagenicity, oncogenicity, and allergenic effects, is studying their influence on gut microbiota. In this paper we describe changes in the composition of rat gut microbiota after six months of consuming one of two recombinant SPs—brazzein or monellin. A full length 16S gene sequencing method was used for DNA library barcoding. The MaAsLin2 analysis results showed noticeable fluctuations in the relative abundances of Anaerocella delicata in brazzein-fed rat microbiota, and of Anaerutruncus rubiinfantis in monellin-fed rat microbiota, which, however, did not exceed the standard deviation. The sucrose-fed group was associated with an increase in the relative abundance of Faecalibaculum rodentium, which may contribute to obesity. Overall, prolonged consumption of the sweet proteins brazzein and monellin did not significantly change rat microbiota and did not result in the appearance of opportunistic microbiota. This provides additional evidence for the safety of these potential sweeteners. |
| first_indexed | 2024-04-24T21:44:13Z |
| format | Article |
| id | doaj.art-e79bc8b6ef6f4ec7ab7af6b5f6995de1 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-24T21:44:13Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-e79bc8b6ef6f4ec7ab7af6b5f6995de12024-03-21T04:33:32ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2024-03-011110.3389/fnut.2024.13625291362529Effect of the consumption of brazzein and monellin, two recombinant sweet-tasting proteins, on rat gut microbiotaVladimir A. Veselovsky0Daria I. Boldyreva1Evgenii I. Olekhnovich2Ksenia M. Klimina3Vladislav V. Babenko4Natalia V. Zakharevich5Andrey K. Larin6Maxim D. Morozov7Polina Y. Zoruk8Petr V. Sergiev9Olga A. Dontsova10Igor V. Maev11Tamara S. Novik12Anatoly A. Kotlobay13Vassili N. Lazarev14Maria A. Lagarkova15Lopukhin Federal Research and Clinical Centre of Physical-Chemical Medicine, Moscow, RussiaLopukhin Federal Research and Clinical Centre of Physical-Chemical Medicine, Moscow, RussiaLopukhin Federal Research and Clinical Centre of Physical-Chemical Medicine, Moscow, RussiaLopukhin Federal Research and Clinical Centre of Physical-Chemical Medicine, Moscow, RussiaLopukhin Federal Research and Clinical Centre of Physical-Chemical Medicine, Moscow, RussiaLopukhin Federal Research and Clinical Centre of Physical-Chemical Medicine, Moscow, RussiaLopukhin Federal Research and Clinical Centre of Physical-Chemical Medicine, Moscow, RussiaLopukhin Federal Research and Clinical Centre of Physical-Chemical Medicine, Moscow, RussiaLopukhin Federal Research and Clinical Centre of Physical-Chemical Medicine, Moscow, RussiaA.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, RussiaA.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, RussiaDepartment of Propaedeutics of Internal Diseases and Gastroenterology, Moscow State University of Medicine and Dentistry, Moscow, RussiaScientific Research Centre Pharmbiomed, Moscow, RussiaLopukhin Federal Research and Clinical Centre of Physical-Chemical Medicine, Moscow, RussiaLopukhin Federal Research and Clinical Centre of Physical-Chemical Medicine, Moscow, RussiaLopukhin Federal Research and Clinical Centre of Physical-Chemical Medicine, Moscow, RussiaSweet-tasting proteins (SPs) are proteins of plant origin initially isolated from tropical fruits. They are thousands of times sweeter than sucrose and most artificial sweeteners. SPs are a class of proteins capable of causing a sweet taste sensation in humans when interacting with the T1R2/T1R3 receptor. SP thaumatin has already been introduced in the food industry in some countries. Other SPs, such as monellin and brazzein, are promising products. An important stage in researching SPs, in addition to confirming the absence of toxicity, mutagenicity, oncogenicity, and allergenic effects, is studying their influence on gut microbiota. In this paper we describe changes in the composition of rat gut microbiota after six months of consuming one of two recombinant SPs—brazzein or monellin. A full length 16S gene sequencing method was used for DNA library barcoding. The MaAsLin2 analysis results showed noticeable fluctuations in the relative abundances of Anaerocella delicata in brazzein-fed rat microbiota, and of Anaerutruncus rubiinfantis in monellin-fed rat microbiota, which, however, did not exceed the standard deviation. The sucrose-fed group was associated with an increase in the relative abundance of Faecalibaculum rodentium, which may contribute to obesity. Overall, prolonged consumption of the sweet proteins brazzein and monellin did not significantly change rat microbiota and did not result in the appearance of opportunistic microbiota. This provides additional evidence for the safety of these potential sweeteners.https://www.frontiersin.org/articles/10.3389/fnut.2024.1362529/fullmicrobiomenatural sweetenersugar substituteintestinal bacteriafood additivesafety |
| spellingShingle | Vladimir A. Veselovsky Daria I. Boldyreva Evgenii I. Olekhnovich Ksenia M. Klimina Vladislav V. Babenko Natalia V. Zakharevich Andrey K. Larin Maxim D. Morozov Polina Y. Zoruk Petr V. Sergiev Olga A. Dontsova Igor V. Maev Tamara S. Novik Anatoly A. Kotlobay Vassili N. Lazarev Maria A. Lagarkova Effect of the consumption of brazzein and monellin, two recombinant sweet-tasting proteins, on rat gut microbiota Frontiers in Nutrition microbiome natural sweetener sugar substitute intestinal bacteria food additive safety |
| title | Effect of the consumption of brazzein and monellin, two recombinant sweet-tasting proteins, on rat gut microbiota |
| title_full | Effect of the consumption of brazzein and monellin, two recombinant sweet-tasting proteins, on rat gut microbiota |
| title_fullStr | Effect of the consumption of brazzein and monellin, two recombinant sweet-tasting proteins, on rat gut microbiota |
| title_full_unstemmed | Effect of the consumption of brazzein and monellin, two recombinant sweet-tasting proteins, on rat gut microbiota |
| title_short | Effect of the consumption of brazzein and monellin, two recombinant sweet-tasting proteins, on rat gut microbiota |
| title_sort | effect of the consumption of brazzein and monellin two recombinant sweet tasting proteins on rat gut microbiota |
| topic | microbiome natural sweetener sugar substitute intestinal bacteria food additive safety |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2024.1362529/full |
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