Adapting an acyl CoA ligase from Metallosphaera sedula for lactam formation by structure-guided protein engineering

Adapting an acyl CoA ligase from Metallosphaera sedula for lactam formation by structure-guided protein engineering

The CoA ligase from Metallosphaera sedula (MsACL) can be used for the chemoenzymatic synthesis of amides from carboxylic acids. In this CoA-independent conversion, the enzyme catalyzes the adenylation of a carboxylic acid with the help of ATP, followed by the uncatalyzed cleavage of acyl-AMP by a nu...

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Bibliographic Details
Main Authors: Nikolas Capra, Chloé Lelièvre, Océane Touré, Aurélie Fossey-Jouenne, Carine Vergne-Vaxelaire, Dick B. Janssen, Andy-Mark W. H. Thunnissen, Anne Zaparucha
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-03-01
Series:Frontiers in Catalysis
Subjects:
CoA ligase
enzyme structure
protein engineering
lactams
chemoenzymatic synthesis
biocatalysis
Online Access:https://www.frontiersin.org/articles/10.3389/fctls.2024.1360129/full

Internet

https://www.frontiersin.org/articles/10.3389/fctls.2024.1360129/full

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