Temperature effect on water dynamics in tetramer phosphofructokinase matrix and the super-arrhenius respiration rate
Abstract Advances in understanding the temperature effect on water dynamics in cellular respiration are important for the modeling of integrated energy processes and metabolic rates. For more than half a century, experimental studies have contributed to the understanding of the catalytic role of wat...
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| Format: | Article |
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Nature Portfolio
2021-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-79271-5 |
| _version_ | 1818858500329046016 |
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| author | Hsiao-Ching Yang Yung-Chi Ge Kuan-Hsuan Su Chia-Cheng Chang King-Chuen Lin Vincenzo Aquilanti Toshio Kasai |
| author_facet | Hsiao-Ching Yang Yung-Chi Ge Kuan-Hsuan Su Chia-Cheng Chang King-Chuen Lin Vincenzo Aquilanti Toshio Kasai |
| author_sort | Hsiao-Ching Yang |
| collection | DOAJ |
| description | Abstract Advances in understanding the temperature effect on water dynamics in cellular respiration are important for the modeling of integrated energy processes and metabolic rates. For more than half a century, experimental studies have contributed to the understanding of the catalytic role of water in respiration combustion, yet the detailed water dynamics remains elusive. We combine a super-Arrhenius model that links the temperature-dependent exponential growth rate of a population of plant cells to respiration, and an experiment on isotope labeled 18O2 uptake to H2 18O transport role and to a rate-limiting step of cellular respiration. We use Phosphofructokinase (PFK-1) as a prototype because this enzyme is known to be a pacemaker (a rate-limiting enzyme) in the glycolysis process of respiration. The characterization shows that PFK-1 water matrix dynamics are crucial for examining how respiration (PFK-1 tetramer complex breathing) rates respond to temperature change through a water and nano-channel network created by the enzyme folding surfaces, at both short and long (evolutionary) timescales. We not only reveal the nano-channel water network of PFK-1 tetramer hydration topography but also clarify how temperature drives the underlying respiration rates by mapping the channels of water diffusion with distinct dynamics in space and time. The results show that the PFK-1 assembly tetramer possesses a sustainable capacity in the regulation of the water network toward metabolic rates. The implications and limitations of the reciprocal-activation–reciprocal-temperature relationship for interpreting PFK-1 tetramer mechanisms are briefly discussed. |
| first_indexed | 2024-12-19T08:57:16Z |
| format | Article |
| id | doaj.art-e7a7b4737c7a4d3384f1296f688d9793 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T08:57:16Z |
| publishDate | 2021-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-e7a7b4737c7a4d3384f1296f688d97932022-12-21T20:28:34ZengNature PortfolioScientific Reports2045-23222021-01-0111111410.1038/s41598-020-79271-5Temperature effect on water dynamics in tetramer phosphofructokinase matrix and the super-arrhenius respiration rateHsiao-Ching Yang0Yung-Chi Ge1Kuan-Hsuan Su2Chia-Cheng Chang3King-Chuen Lin4Vincenzo Aquilanti5Toshio Kasai6Department of Chemistry, Fu Jen Catholic UniversityDepartment of Chemistry, Fu Jen Catholic UniversityDepartment of Chemistry, Fu Jen Catholic UniversityDepartment of Chemistry, Fu Jen Catholic UniversityDepartment of Chemistry, National Taiwan UniversityDipartimento di Chimica, Biologia e Biotecnologie, Università di PerugiaDepartment of Chemistry, National Taiwan UniversityAbstract Advances in understanding the temperature effect on water dynamics in cellular respiration are important for the modeling of integrated energy processes and metabolic rates. For more than half a century, experimental studies have contributed to the understanding of the catalytic role of water in respiration combustion, yet the detailed water dynamics remains elusive. We combine a super-Arrhenius model that links the temperature-dependent exponential growth rate of a population of plant cells to respiration, and an experiment on isotope labeled 18O2 uptake to H2 18O transport role and to a rate-limiting step of cellular respiration. We use Phosphofructokinase (PFK-1) as a prototype because this enzyme is known to be a pacemaker (a rate-limiting enzyme) in the glycolysis process of respiration. The characterization shows that PFK-1 water matrix dynamics are crucial for examining how respiration (PFK-1 tetramer complex breathing) rates respond to temperature change through a water and nano-channel network created by the enzyme folding surfaces, at both short and long (evolutionary) timescales. We not only reveal the nano-channel water network of PFK-1 tetramer hydration topography but also clarify how temperature drives the underlying respiration rates by mapping the channels of water diffusion with distinct dynamics in space and time. The results show that the PFK-1 assembly tetramer possesses a sustainable capacity in the regulation of the water network toward metabolic rates. The implications and limitations of the reciprocal-activation–reciprocal-temperature relationship for interpreting PFK-1 tetramer mechanisms are briefly discussed.https://doi.org/10.1038/s41598-020-79271-5 |
| spellingShingle | Hsiao-Ching Yang Yung-Chi Ge Kuan-Hsuan Su Chia-Cheng Chang King-Chuen Lin Vincenzo Aquilanti Toshio Kasai Temperature effect on water dynamics in tetramer phosphofructokinase matrix and the super-arrhenius respiration rate Scientific Reports |
| title | Temperature effect on water dynamics in tetramer phosphofructokinase matrix and the super-arrhenius respiration rate |
| title_full | Temperature effect on water dynamics in tetramer phosphofructokinase matrix and the super-arrhenius respiration rate |
| title_fullStr | Temperature effect on water dynamics in tetramer phosphofructokinase matrix and the super-arrhenius respiration rate |
| title_full_unstemmed | Temperature effect on water dynamics in tetramer phosphofructokinase matrix and the super-arrhenius respiration rate |
| title_short | Temperature effect on water dynamics in tetramer phosphofructokinase matrix and the super-arrhenius respiration rate |
| title_sort | temperature effect on water dynamics in tetramer phosphofructokinase matrix and the super arrhenius respiration rate |
| url | https://doi.org/10.1038/s41598-020-79271-5 |
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