Gallic acid inhibits osteoclastogenesis and prevents ovariectomy-induced bone loss
Osteoporosis is a common metabolic bone disease with a rapidly increasing prevalence, characterized by massive bone loss because of excessive osteoclast formation. Gallic acid (GA), a phenolic acid isolated from Cornus officinalis, has anti-inflammatory and anti-oxidant effects, but its effect on os...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2022.963237/full |
| _version_ | 1811291296215597056 |
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| author | Peng Zhang Jiekai Ye Jiale Dai Ying Wang Genjun Chen Jinping Hu Qimiao Hu Jun Fei |
| author_facet | Peng Zhang Jiekai Ye Jiale Dai Ying Wang Genjun Chen Jinping Hu Qimiao Hu Jun Fei |
| author_sort | Peng Zhang |
| collection | DOAJ |
| description | Osteoporosis is a common metabolic bone disease with a rapidly increasing prevalence, characterized by massive bone loss because of excessive osteoclast formation. Gallic acid (GA), a phenolic acid isolated from Cornus officinalis, has anti-inflammatory and anti-oxidant effects, but its effect on osteoclast formation has not been confirmed. In our study, we demonstrated that GA significantly inhibited RANKL‐induced osteoclast formation and function of osteoclast in bone marrow monocytes (BMMs) and RAW264.7 cells in a dose-dependent manner without cytotoxicity. For molecular mechanisms, GA repressed osteoclastogenesis by blocking Akt, ERK, and JNK pathways, and suppressed osteoclastogenesis-related marker expression, including nuclear factor of the activated T-cell cytoplasmic 1 (NFATc1), c‐Fos, and cathepsin K (CTSK). In addition, we further assessed the effect of GA in an ovariectomized mouse model, which indicated that GA has a notable effect on preventing bone loss. In conclusion, GA exerts notable effects in inhibiting osteoclastogenesis and preventing ovariectomy-induced bone loss, suggesting that GA is a potential agent in osteoporosis treatment. |
| first_indexed | 2024-04-13T04:27:16Z |
| format | Article |
| id | doaj.art-e7c9bf5635d34b2dabf6623fa0885413 |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-04-13T04:27:16Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-e7c9bf5635d34b2dabf6623fa08854132022-12-22T03:02:28ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922022-12-011310.3389/fendo.2022.963237963237Gallic acid inhibits osteoclastogenesis and prevents ovariectomy-induced bone lossPeng Zhang0Jiekai Ye1Jiale Dai2Ying Wang3Genjun Chen4Jinping Hu5Qimiao Hu6Jun Fei7Zhejiang Hospital of Integrated Traditional Chinese and Western Medicine, Hangzhou, ChinaZhejiang Hospital of Integrated Traditional Chinese and Western Medicine, Hangzhou, ChinaThe Third Clinical College of Zhejiang Chinese Medical University, Hangzhou, ChinaZhejiang Hospital of Integrated Traditional Chinese and Western Medicine, Hangzhou, ChinaZhejiang Hospital of Integrated Traditional Chinese and Western Medicine, Hangzhou, ChinaZhejiang Hospital of Integrated Traditional Chinese and Western Medicine, Hangzhou, ChinaThe Third Clinical College of Zhejiang Chinese Medical University, Hangzhou, ChinaZhejiang Hospital of Integrated Traditional Chinese and Western Medicine, Hangzhou, ChinaOsteoporosis is a common metabolic bone disease with a rapidly increasing prevalence, characterized by massive bone loss because of excessive osteoclast formation. Gallic acid (GA), a phenolic acid isolated from Cornus officinalis, has anti-inflammatory and anti-oxidant effects, but its effect on osteoclast formation has not been confirmed. In our study, we demonstrated that GA significantly inhibited RANKL‐induced osteoclast formation and function of osteoclast in bone marrow monocytes (BMMs) and RAW264.7 cells in a dose-dependent manner without cytotoxicity. For molecular mechanisms, GA repressed osteoclastogenesis by blocking Akt, ERK, and JNK pathways, and suppressed osteoclastogenesis-related marker expression, including nuclear factor of the activated T-cell cytoplasmic 1 (NFATc1), c‐Fos, and cathepsin K (CTSK). In addition, we further assessed the effect of GA in an ovariectomized mouse model, which indicated that GA has a notable effect on preventing bone loss. In conclusion, GA exerts notable effects in inhibiting osteoclastogenesis and preventing ovariectomy-induced bone loss, suggesting that GA is a potential agent in osteoporosis treatment.https://www.frontiersin.org/articles/10.3389/fendo.2022.963237/fullosteoclastGallic acidAktERKJNKosteoporosis |
| spellingShingle | Peng Zhang Jiekai Ye Jiale Dai Ying Wang Genjun Chen Jinping Hu Qimiao Hu Jun Fei Gallic acid inhibits osteoclastogenesis and prevents ovariectomy-induced bone loss Frontiers in Endocrinology osteoclast Gallic acid Akt ERK JNK osteoporosis |
| title | Gallic acid inhibits osteoclastogenesis and prevents ovariectomy-induced bone loss |
| title_full | Gallic acid inhibits osteoclastogenesis and prevents ovariectomy-induced bone loss |
| title_fullStr | Gallic acid inhibits osteoclastogenesis and prevents ovariectomy-induced bone loss |
| title_full_unstemmed | Gallic acid inhibits osteoclastogenesis and prevents ovariectomy-induced bone loss |
| title_short | Gallic acid inhibits osteoclastogenesis and prevents ovariectomy-induced bone loss |
| title_sort | gallic acid inhibits osteoclastogenesis and prevents ovariectomy induced bone loss |
| topic | osteoclast Gallic acid Akt ERK JNK osteoporosis |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2022.963237/full |
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