RNA purification-free detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)

RNA purification-free detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Abstract Background Current diagnosis of SARS-CoV-2 infection relies on RNA purification prior to amplification. Typical extraction methods limit the processing speed and turnaround time for SARS-CoV-2 diagnostic testing. Methods Here, we applied reverse transcription loop-mediated isothermal amplif...

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Bibliographic Details
Main Authors: Meng Yee Lai, Jeyanthi Suppiah, Ravindran Thayan, Ilyiana Ismail, Nur Izati Mustapa, Tuan Suhaila Tuan Soh, Afifah Haji Hassan, Kalaiarasu M. Peariasamy, Yee Leng Lee, Yee Ling Lau
Format: Article
Language:English
Published: BMC 2022-01-01
Series:Tropical Medicine and Health
Subjects:
COVID-19
Nasopharyngeal swab
SARS-CoV-2
RT-LAMP
Online Access:https://doi.org/10.1186/s41182-021-00396-y

Internet

https://doi.org/10.1186/s41182-021-00396-y

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