Optimization of Extraction and Decolorization of Polysaccharides from Spirulina platensis by Response Surface Experiment
The extraction and decolorization conditions for Spirulina platensis polysaccharides using hot alkali and hydrogen peroxide were investigated, and the process was optimized. Based on the results of a single-factor test, the yield and decolorization rate of polysaccharides were taken as indices to op...
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The editorial department of Science and Technology of Food Industry
2024-04-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023050299 |
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| author | Shilin WU Ran CHEN Jingyun CHEN Ning YANG Kun LI Zhen ZHANG Rongqing ZHANG |
| author_facet | Shilin WU Ran CHEN Jingyun CHEN Ning YANG Kun LI Zhen ZHANG Rongqing ZHANG |
| author_sort | Shilin WU |
| collection | DOAJ |
| description | The extraction and decolorization conditions for Spirulina platensis polysaccharides using hot alkali and hydrogen peroxide were investigated, and the process was optimized. Based on the results of a single-factor test, the yield and decolorization rate of polysaccharides were taken as indices to optimize the technological conditions for extracting polysaccharides from Spirulina platensis using the hot alkali method and decolorizing them with hydrogen peroxide using the response surface methodology. The optimal conditions for hot alkali extraction of polysaccharides from Spirulina platensis were determined as follows: NaOH concentration of 1.5%, extraction temperature of 90 ℃, extraction time of 4 hours, and material-to-liquid ratio of 1:20 g/mL. Under these conditions, the yield of polysaccharides from Spirulina platensis was 5.18%±0.23%. The optimal conditions for decolorization using hydrogen peroxide were found to be: 17% hydrogen peroxide solution, decolorization time of 55 minutes, and decolorization temperature of 50 ℃. Under these conditions, the actual decolorization rate was 82.54%±0.03%, and the retention rate of polysaccharides was 83.45%±0.13%. The findings of this study provide valuable insights for the development and utilization of Spirulina platensis polysaccharides. |
| first_indexed | 2024-04-24T20:24:18Z |
| format | Article |
| id | doaj.art-e7ff4d18fd414370be9850e8b670417c |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-24T20:24:18Z |
| publishDate | 2024-04-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
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| series | Shipin gongye ke-ji |
| spelling | doaj.art-e7ff4d18fd414370be9850e8b670417c2024-03-22T06:45:48ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062024-04-0145720120910.13386/j.issn1002-0306.20230502992023050299-7Optimization of Extraction and Decolorization of Polysaccharides from Spirulina platensis by Response Surface ExperimentShilin WU0Ran CHEN1Jingyun CHEN2Ning YANG3Kun LI4Zhen ZHANG5Rongqing ZHANG6College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, ChinaZhejiang Provincial Key Laboratory of Applied Enzymology, Yangtze Delta Region Institute of Tsinghua University, Jiaxing 314000, ChinaThe extraction and decolorization conditions for Spirulina platensis polysaccharides using hot alkali and hydrogen peroxide were investigated, and the process was optimized. Based on the results of a single-factor test, the yield and decolorization rate of polysaccharides were taken as indices to optimize the technological conditions for extracting polysaccharides from Spirulina platensis using the hot alkali method and decolorizing them with hydrogen peroxide using the response surface methodology. The optimal conditions for hot alkali extraction of polysaccharides from Spirulina platensis were determined as follows: NaOH concentration of 1.5%, extraction temperature of 90 ℃, extraction time of 4 hours, and material-to-liquid ratio of 1:20 g/mL. Under these conditions, the yield of polysaccharides from Spirulina platensis was 5.18%±0.23%. The optimal conditions for decolorization using hydrogen peroxide were found to be: 17% hydrogen peroxide solution, decolorization time of 55 minutes, and decolorization temperature of 50 ℃. Under these conditions, the actual decolorization rate was 82.54%±0.03%, and the retention rate of polysaccharides was 83.45%±0.13%. The findings of this study provide valuable insights for the development and utilization of Spirulina platensis polysaccharides.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023050299spirulina platensispolysaccharidehot alkali extractionextractionhydrogen peroxidedecolorization |
| spellingShingle | Shilin WU Ran CHEN Jingyun CHEN Ning YANG Kun LI Zhen ZHANG Rongqing ZHANG Optimization of Extraction and Decolorization of Polysaccharides from Spirulina platensis by Response Surface Experiment Shipin gongye ke-ji spirulina platensis polysaccharide hot alkali extraction extraction hydrogen peroxide decolorization |
| title | Optimization of Extraction and Decolorization of Polysaccharides from Spirulina platensis by Response Surface Experiment |
| title_full | Optimization of Extraction and Decolorization of Polysaccharides from Spirulina platensis by Response Surface Experiment |
| title_fullStr | Optimization of Extraction and Decolorization of Polysaccharides from Spirulina platensis by Response Surface Experiment |
| title_full_unstemmed | Optimization of Extraction and Decolorization of Polysaccharides from Spirulina platensis by Response Surface Experiment |
| title_short | Optimization of Extraction and Decolorization of Polysaccharides from Spirulina platensis by Response Surface Experiment |
| title_sort | optimization of extraction and decolorization of polysaccharides from spirulina platensis by response surface experiment |
| topic | spirulina platensis polysaccharide hot alkali extraction extraction hydrogen peroxide decolorization |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023050299 |
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