Elevated CSF angiopoietin-2 correlates with blood-brain barrier leakiness and markers of neuronal injury in early Alzheimer’s disease
Abstract Breakdown of the neurovascular unit is associated with blood-brain barrier (BBB) leakiness contributing to cognitive decline and disease pathology in the early stages of Alzheimer’s disease (AD). Vascular stability depends on angiopoietin-1 (ANGPT-1) signalling, antagonised by angiopoietin-...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2024-01-01
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| Series: | Translational Psychiatry |
| Online Access: | https://doi.org/10.1038/s41398-023-02706-w |
| _version_ | 1797362883568336896 |
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| author | Carol Van Hulle Selvi Ince Ozioma C. Okonkwo Barbara B. Bendlin Sterling C. Johnson Cynthia M. Carlsson Sanjay Asthana Seth Love Kaj Blennow Henrik Zetterberg J. Scott Miners |
| author_facet | Carol Van Hulle Selvi Ince Ozioma C. Okonkwo Barbara B. Bendlin Sterling C. Johnson Cynthia M. Carlsson Sanjay Asthana Seth Love Kaj Blennow Henrik Zetterberg J. Scott Miners |
| author_sort | Carol Van Hulle |
| collection | DOAJ |
| description | Abstract Breakdown of the neurovascular unit is associated with blood-brain barrier (BBB) leakiness contributing to cognitive decline and disease pathology in the early stages of Alzheimer’s disease (AD). Vascular stability depends on angiopoietin-1 (ANGPT-1) signalling, antagonised by angiopoietin-2 (ANGPT-2) expressed upon endothelial injury. We examined the relationship between CSF ANGPT-2 and CSF markers of BBB leakiness and core AD biomarkers across three independent cohorts: (i) 31 AD patients and 33 healthy controls grouped according to their biomarker profile (i.e., AD cases t-tau > 400 pg/mL, p-tau > 60 pg/mL and Aβ42 < 550 pg/mL); (ii) 121 participants in the Wisconsin Registry for Alzheimer’s Prevention or Wisconsin Alzheimer’s Disease Research study (84 participants cognitively unimpaired (CU) enriched for a parental history of AD, 20 participants with mild cognitive impairment (MCI), and 17 with AD); (iii) a neurologically normal cohort aged 23–78 years with paired CSF and serum samples. CSF ANGPT-2, sPDGFRβ, albumin and fibrinogen levels were measured by sandwich ELISA. In cohort (i), CSF ANGPT-2 was elevated in AD and correlated with CSF t-tau and p-tau181 but not Aβ42. ANGPT-2 also correlated positively with CSF sPDGFRβ and fibrinogen – markers of pericyte injury and BBB leakiness. In cohort (ii), CSF ANGPT-2 was highest in MCI and correlated with CSF albumin in the CU and MCI cohorts but not in AD. CSF ANGPT-2 also correlated with CSF t-tau and p-tau and with markers of neuronal injury (neurogranin and α-synuclein) and neuroinflammation (GFAP and YKL-40). In cohort (iii), CSF ANGPT-2 correlated strongly with the CSF/serum albumin ratio. Serum ANGPT-2 showed non-significant positive associations with CSF ANGPT-2 and the CSF/serum albumin ratio. Together, these data indicate that CSF and possibly serum ANGPT-2 is associated with BBB leakiness in early AD and is closely related to tau pathology and neuronal injury. The utility of serum ANGPT-2 as a biomarker of BBB damage in AD requires further study. |
| first_indexed | 2024-03-08T16:13:50Z |
| format | Article |
| id | doaj.art-e810f28aac8941cab385eb1aa712f3ca |
| institution | Directory Open Access Journal |
| issn | 2158-3188 |
| language | English |
| last_indexed | 2024-03-08T16:13:50Z |
| publishDate | 2024-01-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Translational Psychiatry |
| spelling | doaj.art-e810f28aac8941cab385eb1aa712f3ca2024-01-07T12:48:14ZengNature Publishing GroupTranslational Psychiatry2158-31882024-01-011411810.1038/s41398-023-02706-wElevated CSF angiopoietin-2 correlates with blood-brain barrier leakiness and markers of neuronal injury in early Alzheimer’s diseaseCarol Van Hulle0Selvi Ince1Ozioma C. Okonkwo2Barbara B. Bendlin3Sterling C. Johnson4Cynthia M. Carlsson5Sanjay Asthana6Seth Love7Kaj Blennow8Henrik Zetterberg9J. Scott Miners10Wisconsin Alzheimer’s Disease Research Center, School of Medicine and Public Health, University of Wisconsin-MadisonDementia Research Group, Clinical Neurosciences, Bristol Medical School, University of BristolWisconsin Alzheimer’s Disease Research Center, School of Medicine and Public Health, University of Wisconsin-MadisonWisconsin Alzheimer’s Disease Research Center, School of Medicine and Public Health, University of Wisconsin-MadisonWisconsin Alzheimer’s Disease Research Center, School of Medicine and Public Health, University of Wisconsin-MadisonWisconsin Alzheimer’s Disease Research Center, School of Medicine and Public Health, University of Wisconsin-MadisonWisconsin Alzheimer’s Disease Research Center, School of Medicine and Public Health, University of Wisconsin-MadisonDementia Research Group, Clinical Neurosciences, Bristol Medical School, University of BristolDepartment of Psychiatry and Neurochemistry, Institute of Neuroscience and Physiology, the Sahlgrenska Academy at the University of GothenburgWisconsin Alzheimer’s Disease Research Center, School of Medicine and Public Health, University of Wisconsin-MadisonDementia Research Group, Clinical Neurosciences, Bristol Medical School, University of BristolAbstract Breakdown of the neurovascular unit is associated with blood-brain barrier (BBB) leakiness contributing to cognitive decline and disease pathology in the early stages of Alzheimer’s disease (AD). Vascular stability depends on angiopoietin-1 (ANGPT-1) signalling, antagonised by angiopoietin-2 (ANGPT-2) expressed upon endothelial injury. We examined the relationship between CSF ANGPT-2 and CSF markers of BBB leakiness and core AD biomarkers across three independent cohorts: (i) 31 AD patients and 33 healthy controls grouped according to their biomarker profile (i.e., AD cases t-tau > 400 pg/mL, p-tau > 60 pg/mL and Aβ42 < 550 pg/mL); (ii) 121 participants in the Wisconsin Registry for Alzheimer’s Prevention or Wisconsin Alzheimer’s Disease Research study (84 participants cognitively unimpaired (CU) enriched for a parental history of AD, 20 participants with mild cognitive impairment (MCI), and 17 with AD); (iii) a neurologically normal cohort aged 23–78 years with paired CSF and serum samples. CSF ANGPT-2, sPDGFRβ, albumin and fibrinogen levels were measured by sandwich ELISA. In cohort (i), CSF ANGPT-2 was elevated in AD and correlated with CSF t-tau and p-tau181 but not Aβ42. ANGPT-2 also correlated positively with CSF sPDGFRβ and fibrinogen – markers of pericyte injury and BBB leakiness. In cohort (ii), CSF ANGPT-2 was highest in MCI and correlated with CSF albumin in the CU and MCI cohorts but not in AD. CSF ANGPT-2 also correlated with CSF t-tau and p-tau and with markers of neuronal injury (neurogranin and α-synuclein) and neuroinflammation (GFAP and YKL-40). In cohort (iii), CSF ANGPT-2 correlated strongly with the CSF/serum albumin ratio. Serum ANGPT-2 showed non-significant positive associations with CSF ANGPT-2 and the CSF/serum albumin ratio. Together, these data indicate that CSF and possibly serum ANGPT-2 is associated with BBB leakiness in early AD and is closely related to tau pathology and neuronal injury. The utility of serum ANGPT-2 as a biomarker of BBB damage in AD requires further study.https://doi.org/10.1038/s41398-023-02706-w |
| spellingShingle | Carol Van Hulle Selvi Ince Ozioma C. Okonkwo Barbara B. Bendlin Sterling C. Johnson Cynthia M. Carlsson Sanjay Asthana Seth Love Kaj Blennow Henrik Zetterberg J. Scott Miners Elevated CSF angiopoietin-2 correlates with blood-brain barrier leakiness and markers of neuronal injury in early Alzheimer’s disease Translational Psychiatry |
| title | Elevated CSF angiopoietin-2 correlates with blood-brain barrier leakiness and markers of neuronal injury in early Alzheimer’s disease |
| title_full | Elevated CSF angiopoietin-2 correlates with blood-brain barrier leakiness and markers of neuronal injury in early Alzheimer’s disease |
| title_fullStr | Elevated CSF angiopoietin-2 correlates with blood-brain barrier leakiness and markers of neuronal injury in early Alzheimer’s disease |
| title_full_unstemmed | Elevated CSF angiopoietin-2 correlates with blood-brain barrier leakiness and markers of neuronal injury in early Alzheimer’s disease |
| title_short | Elevated CSF angiopoietin-2 correlates with blood-brain barrier leakiness and markers of neuronal injury in early Alzheimer’s disease |
| title_sort | elevated csf angiopoietin 2 correlates with blood brain barrier leakiness and markers of neuronal injury in early alzheimer s disease |
| url | https://doi.org/10.1038/s41398-023-02706-w |
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