Establishment of porcine nuclear transfer-derived embryonic stem cells using induced pluripotent stem cells as donor nuclei

We investigated whether sequential reprogramming via porcine induced pluripotent stem cells (piPSCs) or exposure to oocyte cytoplasm following nuclear transfer could generate nuclear transfer-derived ESCs (piPSCs-ntESCs). Nuclear transfer embryos were reconstructed with piPSCs possessing a ZsGreen f...

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Bibliographic Details
Main Authors: Seiki HARAGUCHI, Thanh Quang DANG-NGUYEN, David WELLS, Daiichiro FUCHIMOTO, Tomokazu FUKUDA, Tomoyuki TOKUNAGA
Format: Article
Language:English
Published: The Society for Reproduction and Development 2020-01-01
Series:The Journal of Reproduction and Development
Subjects:
Online Access:https://www.jstage.jst.go.jp/article/jrd/66/2/66_2019-137/_pdf/-char/en