Comprehensive analysis of M2 macrophage-derived exosomes facilitating osteogenic differentiation of human periodontal ligament stem cells
Abstract Background The role of periodontal ligament stem cells (PDLSCs) and macrophage polarization in periodontal tissue regeneration and bone remodeling during orthodontic tooth movement (OTM) has been well documented. Nevertheless, the interactions between macrophages and PDLSCs in OTM remain to...
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| Format: | Article |
| Language: | English |
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BMC
2022-12-01
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| Series: | BMC Oral Health |
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| Online Access: | https://doi.org/10.1186/s12903-022-02682-5 |
| _version_ | 1797973460698267648 |
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| author | Xian-min Liao Zheng Guan Zhen-jin Yang Li-ya Ma Ying-juan Dai Cun Liang Jiang-tian Hu |
| author_facet | Xian-min Liao Zheng Guan Zhen-jin Yang Li-ya Ma Ying-juan Dai Cun Liang Jiang-tian Hu |
| author_sort | Xian-min Liao |
| collection | DOAJ |
| description | Abstract Background The role of periodontal ligament stem cells (PDLSCs) and macrophage polarization in periodontal tissue regeneration and bone remodeling during orthodontic tooth movement (OTM) has been well documented. Nevertheless, the interactions between macrophages and PDLSCs in OTM remain to be investigated. Consequently, the present study was proposed to explore the effect of different polarization states of macrophages on the osteogenic differentiation of PDLSCs. Methods After M0, M1 and M2 macrophage-derived exosomes (M0-exo, M1-exo and M2-exo) treatment of primary cultured human PDLSCs, respectively, mineralized nodules were observed by Alizarin red S staining, and the expression of ALP and OCN mRNA and protein were detected by RT-qPCR and Western blotting, correspondingly. Identification of differentially expressed microRNAs (DE-miRNA) in M0-exo and M2-exo by miRNA microarray, and GO and KEGG enrichment analysis of DE-miRNA targets, and construction of protein–protein interaction networks. Results M2-exo augmented mineralized nodule formation and upregulated ALP and OCN expression in PDLSCs, while M0-exo had no significant effect. Compared to M0-exo, a total of 52 DE-miRNAs were identified in M2-exo. The expression of hsa-miR-6507-3p, hsa-miR-4731-3p, hsa-miR-4728-3p, hsa-miR-3614-5p and hsa-miR-6785-3p was significantly down-regulated, and the expression of hsa-miR-6085, hsa-miR-4800-5p, hsa-miR-4778-5p, hsa-miR-6780b-5p and hsa-miR-1227-5p was significantly up-regulated in M2-exo compared to M0-exo. GO and KEGG enrichment analysis revealed that the downstream targets of DE-miRNAs were mainly involved in the differentiation and migration of multiple cells. Conclusions In summary, we have indicated for the first time that M2-exo can promote osteogenic differentiation of human PDLSCs, and have revealed the functions and pathways involved in the DE-miRNAs of M0-exo and M2-exo and their downstream targets. |
| first_indexed | 2024-04-11T04:04:38Z |
| format | Article |
| id | doaj.art-e8323252ec8b467f83cf8effd1f8c3fb |
| institution | Directory Open Access Journal |
| issn | 1472-6831 |
| language | English |
| last_indexed | 2024-04-11T04:04:38Z |
| publishDate | 2022-12-01 |
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| series | BMC Oral Health |
| spelling | doaj.art-e8323252ec8b467f83cf8effd1f8c3fb2023-01-01T12:29:11ZengBMCBMC Oral Health1472-68312022-12-0122111410.1186/s12903-022-02682-5Comprehensive analysis of M2 macrophage-derived exosomes facilitating osteogenic differentiation of human periodontal ligament stem cellsXian-min Liao0Zheng Guan1Zhen-jin Yang2Li-ya Ma3Ying-juan Dai4Cun Liang5Jiang-tian Hu6Department of Orthodontics, Hospital of Stomatology, Kunming Medical University/Yunnan Stomatology HospitalBiomedical Research Center, Affiliated Calmette Hospital of Kunming Medical University/the First Hospital of KunmingDepartment of Orthodontics, Hospital of Stomatology, Kunming Medical University/Yunnan Stomatology HospitalDepartment of Orthodontics, Hospital of Stomatology, Kunming Medical University/Yunnan Stomatology HospitalDepartment of Orthodontics, Hospital of Stomatology, Kunming Medical University/Yunnan Stomatology HospitalDepartment of Orthodontics, Hospital of Stomatology, Kunming Medical University/Yunnan Stomatology HospitalDepartment of Orthodontics, Hospital of Stomatology, Kunming Medical University/Yunnan Stomatology HospitalAbstract Background The role of periodontal ligament stem cells (PDLSCs) and macrophage polarization in periodontal tissue regeneration and bone remodeling during orthodontic tooth movement (OTM) has been well documented. Nevertheless, the interactions between macrophages and PDLSCs in OTM remain to be investigated. Consequently, the present study was proposed to explore the effect of different polarization states of macrophages on the osteogenic differentiation of PDLSCs. Methods After M0, M1 and M2 macrophage-derived exosomes (M0-exo, M1-exo and M2-exo) treatment of primary cultured human PDLSCs, respectively, mineralized nodules were observed by Alizarin red S staining, and the expression of ALP and OCN mRNA and protein were detected by RT-qPCR and Western blotting, correspondingly. Identification of differentially expressed microRNAs (DE-miRNA) in M0-exo and M2-exo by miRNA microarray, and GO and KEGG enrichment analysis of DE-miRNA targets, and construction of protein–protein interaction networks. Results M2-exo augmented mineralized nodule formation and upregulated ALP and OCN expression in PDLSCs, while M0-exo had no significant effect. Compared to M0-exo, a total of 52 DE-miRNAs were identified in M2-exo. The expression of hsa-miR-6507-3p, hsa-miR-4731-3p, hsa-miR-4728-3p, hsa-miR-3614-5p and hsa-miR-6785-3p was significantly down-regulated, and the expression of hsa-miR-6085, hsa-miR-4800-5p, hsa-miR-4778-5p, hsa-miR-6780b-5p and hsa-miR-1227-5p was significantly up-regulated in M2-exo compared to M0-exo. GO and KEGG enrichment analysis revealed that the downstream targets of DE-miRNAs were mainly involved in the differentiation and migration of multiple cells. Conclusions In summary, we have indicated for the first time that M2-exo can promote osteogenic differentiation of human PDLSCs, and have revealed the functions and pathways involved in the DE-miRNAs of M0-exo and M2-exo and their downstream targets.https://doi.org/10.1186/s12903-022-02682-5Orthodontic tooth movementPeriodontal ligament stem cellsMacrophage polarizationExosome, microRNA |
| spellingShingle | Xian-min Liao Zheng Guan Zhen-jin Yang Li-ya Ma Ying-juan Dai Cun Liang Jiang-tian Hu Comprehensive analysis of M2 macrophage-derived exosomes facilitating osteogenic differentiation of human periodontal ligament stem cells BMC Oral Health Orthodontic tooth movement Periodontal ligament stem cells Macrophage polarization Exosome, microRNA |
| title | Comprehensive analysis of M2 macrophage-derived exosomes facilitating osteogenic differentiation of human periodontal ligament stem cells |
| title_full | Comprehensive analysis of M2 macrophage-derived exosomes facilitating osteogenic differentiation of human periodontal ligament stem cells |
| title_fullStr | Comprehensive analysis of M2 macrophage-derived exosomes facilitating osteogenic differentiation of human periodontal ligament stem cells |
| title_full_unstemmed | Comprehensive analysis of M2 macrophage-derived exosomes facilitating osteogenic differentiation of human periodontal ligament stem cells |
| title_short | Comprehensive analysis of M2 macrophage-derived exosomes facilitating osteogenic differentiation of human periodontal ligament stem cells |
| title_sort | comprehensive analysis of m2 macrophage derived exosomes facilitating osteogenic differentiation of human periodontal ligament stem cells |
| topic | Orthodontic tooth movement Periodontal ligament stem cells Macrophage polarization Exosome, microRNA |
| url | https://doi.org/10.1186/s12903-022-02682-5 |
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