Solid-phase combinatorial synthesis using microarrays of microcompartments with light-induced on-chip cell screening
The process of drug discovery includes individual synthesis and characterization of drug candidates, followed by a biological screening, which is separated from synthesis in space and time. This approach suffers from low throughput and associated high costs, which in turn lead to inefficiency in the...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-06-01
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| Series: | Materials Today Bio |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006419300481 |
| _version_ | 1828365597274013696 |
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| author | A. Rosenfeld M. Brehm A. Welle V. Trouillet S. Heissler M. Benz P.A. Levkin |
| author_facet | A. Rosenfeld M. Brehm A. Welle V. Trouillet S. Heissler M. Benz P.A. Levkin |
| author_sort | A. Rosenfeld |
| collection | DOAJ |
| description | The process of drug discovery includes individual synthesis and characterization of drug candidates, followed by a biological screening, which is separated from synthesis in space and time. This approach suffers from low throughput and associated high costs, which in turn lead to inefficiency in the field of drug discovery. Here, we present a miniaturized platform combining combinatorial solid-phase synthesis with high-throughput cell screenings. The method is based on the formation of nanoporous poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) layers patterned with hydrophilic spots separated from each other by superhydrophobic liquid-impermeable barriers. The porous polymer inside the hydrophilic spots is used as a support to conduct solid-phase synthesis. The hydrophilic spots can be then filled with droplets containing either reagents for synthesis or live cells. Upon irradiation with UV light, products of solid-phase synthesis are released from the porous polymer because of the photo-cleavable linkers used and diffuse into separate droplets. The light-induced release of the products allows the control of the release spatially, temporally, and quantitatively. To demonstrate the versatility and usability of the platform for various cell lines, we have successfully implemented peptide synthesis to create an exemplary chemical library and demonstrated high cell viability after the UV-triggered small-molecule release. Keywords: Miniaturization, High-throughput screening, Combinatorial library, Photolytic release |
| first_indexed | 2024-04-14T05:33:21Z |
| format | Article |
| id | doaj.art-e836cf7d8b6546b8aeb378d47e40f18f |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-04-14T05:33:21Z |
| publishDate | 2019-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-e836cf7d8b6546b8aeb378d47e40f18f2022-12-22T02:09:44ZengElsevierMaterials Today Bio2590-00642019-06-013Solid-phase combinatorial synthesis using microarrays of microcompartments with light-induced on-chip cell screeningA. Rosenfeld0M. Brehm1A. Welle2V. Trouillet3S. Heissler4M. Benz5P.A. Levkin6Karlsruhe Institute of Technology (KIT), Institute of Toxicology and Genetics (ITG), Hermann-von Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, GermanyKarlsruhe Institute of Technology (KIT), Institute of Toxicology and Genetics (ITG), Hermann-von Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, GermanyKarlsruhe Institute of Technology (KIT), Institute of Functional Interfaces, Hermann-von Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany; Karlsruhe Institute of Technology (KIT), Karlsruhe Nano Micro Facility, Hermann-von Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, GermanyKarlsruhe Institute of Technology (KIT), Karlsruhe Nano Micro Facility, Hermann-von Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany; Karlsruhe Institute of Technology (KIT), Institute for Applied Materials, Hermann-von Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, GermanyKarlsruhe Institute of Technology (KIT), Institute of Functional Interfaces, Hermann-von Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, GermanyKarlsruhe Institute of Technology (KIT), Institute of Toxicology and Genetics (ITG), Hermann-von Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, GermanyKarlsruhe Institute of Technology (KIT), Institute of Toxicology and Genetics (ITG), Hermann-von Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany; Karlsruhe Institute of Technology (KIT), Institute of Organic Chemistry, 76131, Karlsruhe, Germany; Corresponding author. Karlsruhe Institute of Technology (KIT), Institute of Toxicology and Genetics (ITG), Hermann-von Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.The process of drug discovery includes individual synthesis and characterization of drug candidates, followed by a biological screening, which is separated from synthesis in space and time. This approach suffers from low throughput and associated high costs, which in turn lead to inefficiency in the field of drug discovery. Here, we present a miniaturized platform combining combinatorial solid-phase synthesis with high-throughput cell screenings. The method is based on the formation of nanoporous poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) layers patterned with hydrophilic spots separated from each other by superhydrophobic liquid-impermeable barriers. The porous polymer inside the hydrophilic spots is used as a support to conduct solid-phase synthesis. The hydrophilic spots can be then filled with droplets containing either reagents for synthesis or live cells. Upon irradiation with UV light, products of solid-phase synthesis are released from the porous polymer because of the photo-cleavable linkers used and diffuse into separate droplets. The light-induced release of the products allows the control of the release spatially, temporally, and quantitatively. To demonstrate the versatility and usability of the platform for various cell lines, we have successfully implemented peptide synthesis to create an exemplary chemical library and demonstrated high cell viability after the UV-triggered small-molecule release. Keywords: Miniaturization, High-throughput screening, Combinatorial library, Photolytic releasehttp://www.sciencedirect.com/science/article/pii/S2590006419300481 |
| spellingShingle | A. Rosenfeld M. Brehm A. Welle V. Trouillet S. Heissler M. Benz P.A. Levkin Solid-phase combinatorial synthesis using microarrays of microcompartments with light-induced on-chip cell screening Materials Today Bio |
| title | Solid-phase combinatorial synthesis using microarrays of microcompartments with light-induced on-chip cell screening |
| title_full | Solid-phase combinatorial synthesis using microarrays of microcompartments with light-induced on-chip cell screening |
| title_fullStr | Solid-phase combinatorial synthesis using microarrays of microcompartments with light-induced on-chip cell screening |
| title_full_unstemmed | Solid-phase combinatorial synthesis using microarrays of microcompartments with light-induced on-chip cell screening |
| title_short | Solid-phase combinatorial synthesis using microarrays of microcompartments with light-induced on-chip cell screening |
| title_sort | solid phase combinatorial synthesis using microarrays of microcompartments with light induced on chip cell screening |
| url | http://www.sciencedirect.com/science/article/pii/S2590006419300481 |
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