3D dynamic observation of human sperm by parallel phase-shifting digital holographic microscopy based on pixelated polarization

Morphology and motility are essential criteria for assessing sperm viability. However, the human sperm head is small (∼3–4 μm) and requires a relatively high-magnification microscope objective, while the sperm flagella (∼45 μm) are poorly visible with complex 3D properties. Microscopic dynamic obser...

Full description

Bibliographic Details
Main Authors: Chuanbiao Bai, Zhaoxiang Jiang, Jiangcheng Zhao, Shangquan Wu, Qingchuan Zhang
Format: Article
Language:English
Published: AIP Publishing LLC 2023-07-01
Series:APL Photonics
Online Access:http://dx.doi.org/10.1063/5.0150582