3D dynamic observation of human sperm by parallel phase-shifting digital holographic microscopy based on pixelated polarization
Morphology and motility are essential criteria for assessing sperm viability. However, the human sperm head is small (∼3–4 μm) and requires a relatively high-magnification microscope objective, while the sperm flagella (∼45 μm) are poorly visible with complex 3D properties. Microscopic dynamic obser...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
AIP Publishing LLC
2023-07-01
|
Series: | APL Photonics |
Online Access: | http://dx.doi.org/10.1063/5.0150582 |