Infusion of C20:0 ceramide into ventral hippocampus triggers anhedonia-like behavior in female and male rats
Increased long-chain C20:0 ceramides have been found in the serum of patients with depression. Moreover, ceramides are linked with increased microglia reactivity and inflammatory cytokine production, which are associated with depression. Since ceramides can readily cross the blood brain barrier, per...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Behavioral Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnbeh.2022.899627/full |
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| author | Lubriel Sambolín-Escobales Adariana Feliciano-Quiñones Lizmarie Tirado-Castro Cristina Suárez Dariangelly Pacheco-Cruz Nashaly Irizarry-Méndez Wilfred Fonseca-Ferrer Anixa Hernández-López María Colón-Romero James T. Porter |
| author_facet | Lubriel Sambolín-Escobales Adariana Feliciano-Quiñones Lizmarie Tirado-Castro Cristina Suárez Dariangelly Pacheco-Cruz Nashaly Irizarry-Méndez Wilfred Fonseca-Ferrer Anixa Hernández-López María Colón-Romero James T. Porter |
| author_sort | Lubriel Sambolín-Escobales |
| collection | DOAJ |
| description | Increased long-chain C20:0 ceramides have been found in the serum of patients with depression. Moreover, ceramides are linked with increased microglia reactivity and inflammatory cytokine production, which are associated with depression. Since ceramides can readily cross the blood brain barrier, peripheral C20:0 ceramides could enter the brain, activate microglia, and induce depressive-like behavior. In this study, we determined whether localized infusion of C20:0 ceramides into the ventral hippocampus (VH) of rats is sufficient to activate microglia and induce depressive-like behaviors. Adult male and female rats received infusions of C20:0 ceramides or vehicle solution every other day for 2 weeks. After the third infusion, C20:0-infused animals showed reduced sucrose preference suggesting anhedonia-like behavior. In contrast, infusions of C20:0 ceramides did not affect immobility in the forced swim test or sucrose grooming suggesting that the behavioral effects of ceramides are task dependent. Furthermore, C20:0-infusions did not increase Iba-1 + microglia or inflammatory markers in the VH suggesting that localized increases in C20:0 ceramides in the VH are sufficient to induce anhedonia-like behavior without microglia activation. |
| first_indexed | 2024-12-10T23:57:33Z |
| format | Article |
| id | doaj.art-e86bca54c28d451480934eecc91d6133 |
| institution | Directory Open Access Journal |
| issn | 1662-5153 |
| language | English |
| last_indexed | 2024-12-10T23:57:33Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Behavioral Neuroscience |
| spelling | doaj.art-e86bca54c28d451480934eecc91d61332022-12-22T01:28:33ZengFrontiers Media S.A.Frontiers in Behavioral Neuroscience1662-51532022-08-011610.3389/fnbeh.2022.899627899627Infusion of C20:0 ceramide into ventral hippocampus triggers anhedonia-like behavior in female and male ratsLubriel Sambolín-Escobales0Adariana Feliciano-Quiñones1Lizmarie Tirado-Castro2Cristina Suárez3Dariangelly Pacheco-Cruz4Nashaly Irizarry-Méndez5Wilfred Fonseca-Ferrer6Anixa Hernández-López7María Colón-Romero8James T. Porter9Division of Pharmacology, Basic Sciences Department, Ponce Research Institute, Ponce Health Sciences University, Ponce, Puerto RicoDivision of Pharmacology, Basic Sciences Department, Ponce Research Institute, Ponce Health Sciences University, Ponce, Puerto RicoDivision of Pharmacology, Basic Sciences Department, Ponce Research Institute, Ponce Health Sciences University, Ponce, Puerto RicoDivision of Pharmacology, Basic Sciences Department, Ponce Research Institute, Ponce Health Sciences University, Ponce, Puerto RicoBiology and Biotechnology Department, Pontifical Catholic University of Puerto Rico, Ponce, Puerto RicoDivision of Pharmacology, Basic Sciences Department, Ponce Research Institute, Ponce Health Sciences University, Ponce, Puerto RicoBiology Department, University of Puerto Rico, Ponce, Puerto RicoDivision of Pharmacology, Basic Sciences Department, Ponce Research Institute, Ponce Health Sciences University, Ponce, Puerto RicoDivision of Pharmacology, Basic Sciences Department, Ponce Research Institute, Ponce Health Sciences University, Ponce, Puerto RicoDivision of Pharmacology, Basic Sciences Department, Ponce Research Institute, Ponce Health Sciences University, Ponce, Puerto RicoIncreased long-chain C20:0 ceramides have been found in the serum of patients with depression. Moreover, ceramides are linked with increased microglia reactivity and inflammatory cytokine production, which are associated with depression. Since ceramides can readily cross the blood brain barrier, peripheral C20:0 ceramides could enter the brain, activate microglia, and induce depressive-like behavior. In this study, we determined whether localized infusion of C20:0 ceramides into the ventral hippocampus (VH) of rats is sufficient to activate microglia and induce depressive-like behaviors. Adult male and female rats received infusions of C20:0 ceramides or vehicle solution every other day for 2 weeks. After the third infusion, C20:0-infused animals showed reduced sucrose preference suggesting anhedonia-like behavior. In contrast, infusions of C20:0 ceramides did not affect immobility in the forced swim test or sucrose grooming suggesting that the behavioral effects of ceramides are task dependent. Furthermore, C20:0-infusions did not increase Iba-1 + microglia or inflammatory markers in the VH suggesting that localized increases in C20:0 ceramides in the VH are sufficient to induce anhedonia-like behavior without microglia activation.https://www.frontiersin.org/articles/10.3389/fnbeh.2022.899627/fullceramidesanhedonia modelmicrogliaventral hippocampus (VH)despair swim test |
| spellingShingle | Lubriel Sambolín-Escobales Adariana Feliciano-Quiñones Lizmarie Tirado-Castro Cristina Suárez Dariangelly Pacheco-Cruz Nashaly Irizarry-Méndez Wilfred Fonseca-Ferrer Anixa Hernández-López María Colón-Romero James T. Porter Infusion of C20:0 ceramide into ventral hippocampus triggers anhedonia-like behavior in female and male rats Frontiers in Behavioral Neuroscience ceramides anhedonia model microglia ventral hippocampus (VH) despair swim test |
| title | Infusion of C20:0 ceramide into ventral hippocampus triggers anhedonia-like behavior in female and male rats |
| title_full | Infusion of C20:0 ceramide into ventral hippocampus triggers anhedonia-like behavior in female and male rats |
| title_fullStr | Infusion of C20:0 ceramide into ventral hippocampus triggers anhedonia-like behavior in female and male rats |
| title_full_unstemmed | Infusion of C20:0 ceramide into ventral hippocampus triggers anhedonia-like behavior in female and male rats |
| title_short | Infusion of C20:0 ceramide into ventral hippocampus triggers anhedonia-like behavior in female and male rats |
| title_sort | infusion of c20 0 ceramide into ventral hippocampus triggers anhedonia like behavior in female and male rats |
| topic | ceramides anhedonia model microglia ventral hippocampus (VH) despair swim test |
| url | https://www.frontiersin.org/articles/10.3389/fnbeh.2022.899627/full |
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