Generation of three TTN knock-out human induced pluripotent stem cell lines using CRISPR/Cas9 system
TTN mutations are the common genetic cause for various types of cardiomyopathies (e.g., dilated cardiomyopathy, hypertrophic cardiomyopathy, restrictive cardiomyopathy, and arrhythmogenic right ventricular cardiomyopathy) and skeletal myopathies. Here, we generated three TTN knock-out human induced...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-10-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506122002501 |
| _version_ | 1828322264400003072 |
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| author | Ji-Young Kang Dasom Mun Yumin Chun Da-Seul Park Hyoeun Kim Nuri Yun Seung-Hyun Lee Boyoung Joung |
| author_facet | Ji-Young Kang Dasom Mun Yumin Chun Da-Seul Park Hyoeun Kim Nuri Yun Seung-Hyun Lee Boyoung Joung |
| author_sort | Ji-Young Kang |
| collection | DOAJ |
| description | TTN mutations are the common genetic cause for various types of cardiomyopathies (e.g., dilated cardiomyopathy, hypertrophic cardiomyopathy, restrictive cardiomyopathy, and arrhythmogenic right ventricular cardiomyopathy) and skeletal myopathies. Here, we generated three TTN knock-out human induced pluripotent stem cell (iPSC) lines using CRISPR/Cas9 system. These cell lines, which exhibit normal karyotype, typical morphology and pluripotency, could provide useful platform for investigating the role of TTN in associated disorders. |
| first_indexed | 2024-04-13T18:38:26Z |
| format | Article |
| id | doaj.art-e8b63f964ebf401b8c6c3f49883d1327 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-04-13T18:38:26Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-e8b63f964ebf401b8c6c3f49883d13272022-12-22T02:34:49ZengElsevierStem Cell Research1873-50612022-10-0164102901Generation of three TTN knock-out human induced pluripotent stem cell lines using CRISPR/Cas9 systemJi-Young Kang0Dasom Mun1Yumin Chun2Da-Seul Park3Hyoeun Kim4Nuri Yun5Seung-Hyun Lee6Boyoung Joung7Division of Cardiology, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 03722, South KoreaDivision of Cardiology, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 03722, South KoreaDivision of Cardiology, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 03722, South KoreaDivision of Cardiology, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 03722, South KoreaDepartment of Biochemistry and Molecular Biology, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 03722, South KoreaInstitute of Life Science & Biotechnology, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, South KoreaDepartment of Biochemistry and Molecular Biology, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 03722, South Korea; Corresponding authors.Division of Cardiology, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 03722, South Korea; Corresponding authors.TTN mutations are the common genetic cause for various types of cardiomyopathies (e.g., dilated cardiomyopathy, hypertrophic cardiomyopathy, restrictive cardiomyopathy, and arrhythmogenic right ventricular cardiomyopathy) and skeletal myopathies. Here, we generated three TTN knock-out human induced pluripotent stem cell (iPSC) lines using CRISPR/Cas9 system. These cell lines, which exhibit normal karyotype, typical morphology and pluripotency, could provide useful platform for investigating the role of TTN in associated disorders.http://www.sciencedirect.com/science/article/pii/S1873506122002501 |
| spellingShingle | Ji-Young Kang Dasom Mun Yumin Chun Da-Seul Park Hyoeun Kim Nuri Yun Seung-Hyun Lee Boyoung Joung Generation of three TTN knock-out human induced pluripotent stem cell lines using CRISPR/Cas9 system Stem Cell Research |
| title | Generation of three TTN knock-out human induced pluripotent stem cell lines using CRISPR/Cas9 system |
| title_full | Generation of three TTN knock-out human induced pluripotent stem cell lines using CRISPR/Cas9 system |
| title_fullStr | Generation of three TTN knock-out human induced pluripotent stem cell lines using CRISPR/Cas9 system |
| title_full_unstemmed | Generation of three TTN knock-out human induced pluripotent stem cell lines using CRISPR/Cas9 system |
| title_short | Generation of three TTN knock-out human induced pluripotent stem cell lines using CRISPR/Cas9 system |
| title_sort | generation of three ttn knock out human induced pluripotent stem cell lines using crispr cas9 system |
| url | http://www.sciencedirect.com/science/article/pii/S1873506122002501 |
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