Development and Validation of a New DIVA Real-Time PCR Allowing to Differentiate Wild-Type Lumpy Skin Disease Virus Strains, Including the Asian Recombinant Strains, from Neethling-Based Vaccine Strains

The current epidemic in Asia, driven by LSDV recombinants, poses difficulties to existing DIVA PCR tests, as these do not differentiate between homologous vaccine strains and the recombinant strains. We, therefore, developed and validated a new duplex real-time PCR capable of differentiating Neethli...

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Bibliographic Details
Main Authors: Andy Haegeman, Ilse De Leeuw, Wannes Philips, Nick De Regge
Format: Article
Language:English
Published: MDPI AG 2023-03-01
Series:Viruses
Subjects:
Online Access:https://www.mdpi.com/1999-4915/15/4/870