The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice

The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice

Auxin-inducible degron systems can be leaky and require high doses of auxin. Here the authors establish AID2 which uses an OsTIR1 mutant and the ligand 5-Ph-IAA to overcome these problems and establish AID-mediated target depletion in mice.

Bibliographic Details
Main Authors: Aisha Yesbolatova, Yuichiro Saito, Naomi Kitamoto, Hatsune Makino-Itou, Rieko Ajima, Risako Nakano, Hirofumi Nakaoka, Kosuke Fukui, Kanae Gamo, Yusuke Tominari, Haruki Takeuchi, Yumiko Saga, Ken-ichiro Hayashi, Masato T. Kanemaki
Format: Article
Language:English
Published: Nature Portfolio 2020-11-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-19532-z

Internet

https://doi.org/10.1038/s41467-020-19532-z

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