Mitochondrial Impairment May Increase Cellular NAD(P)H: Resazurin Oxidoreductase Activity, Perturbing the NAD(P)H-Based Viability Assays

Mitochondrial Impairment May Increase Cellular NAD(P)H: Resazurin Oxidoreductase Activity, Perturbing the NAD(P)H-Based Viability Assays

Cellular NAD(P)H-dependent oxidoreductase activity with artificial dyes (NAD(P)H-OR) is an indicator of viability, as the cellular redox state is important for biosynthesis and antioxidant defense. However, high NAD(P)H due to impaired mitochondrial oxidation, known as reductive stress, should incre...

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Bibliographic Details
Main Authors: Vasily A. Aleshin, Artem V. Artiukhov, Henry Oppermann, Alexey V. Kazantsev, Nikolay V. Lukashev, Victoria I. Bunik
Format: Article
Language:English
Published: MDPI AG 2015-08-01
Series:Cells
Subjects:
glioblastoma viability
cellular NAD(P)H-dependent oxidoreductase
metabolon
thiamin
oxythiamin
2-oxo acid dehydrogenase
phosphonate analog of 2-oxo acid
resazurin
T98G
U87
Online Access:http://www.mdpi.com/2073-4409/4/3/427

Internet

http://www.mdpi.com/2073-4409/4/3/427

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