Mitochondrial Impairment May Increase Cellular NAD(P)H: Resazurin Oxidoreductase Activity, Perturbing the NAD(P)H-Based Viability Assays
Cellular NAD(P)H-dependent oxidoreductase activity with artificial dyes (NAD(P)H-OR) is an indicator of viability, as the cellular redox state is important for biosynthesis and antioxidant defense. However, high NAD(P)H due to impaired mitochondrial oxidation, known as reductive stress, should incre...
Main Authors: | Vasily A. Aleshin, Artem V. Artiukhov, Henry Oppermann, Alexey V. Kazantsev, Nikolay V. Lukashev, Victoria I. Bunik |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2015-08-01
|
Series: | Cells |
Subjects: | |
Online Access: | http://www.mdpi.com/2073-4409/4/3/427 |
Similar Items
-
Administration of Phosphonate Inhibitors of Dehydrogenases of 2-Oxoglutarate and 2-Oxoadipate to Rats Elicits Target-Specific Metabolic and Physiological Responses
by: Victoria I. Bunik, et al.
Published: (2022-06-01) -
Phosphonate Inhibitors of Pyruvate Dehydrogenase Perturb Homeostasis of Amino Acids and Protein Succinylation in the Brain
by: Artem V. Artiukhov, et al.
Published: (2022-10-01) -
A New Method to Determine Antioxidant Activities of Biofilms Using a pH Indicator (Resazurin) Model System
by: Young-Teck Kim, et al.
Published: (2023-02-01) -
Roles of NAD(P)H:quinone Oxidoreductase 1 in Diverse Diseases
by: Wang-Soo Lee, et al.
Published: (2021-11-01) -
Chemistry and application of H-phosphonates /
by: Troev, Kolio D. (Kolio Dimov), 1944-
Published: (2006)