Dual Fluorescence Splicing Reporter Minigene Identifies an Antisense Oligonucleotide to Skip Exon v8 of the <i>CD44</i> Gene

Dual Fluorescence Splicing Reporter Minigene Identifies an Antisense Oligonucleotide to Skip Exon v8 of the <i>CD44</i> Gene

Splicing reporter minigenes are used in cell-based in vitro splicing studies. Exon skippable antisense oligonucleotide (ASO) has been identified using minigene splicing assays, but these assays include a time- and cost-consuming step of reverse transcription PCR amplification. To make in vitro splic...

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Bibliographic Details
Main Authors: Sachiyo Fukushima, Manal Farea, Kazuhiro Maeta, Abdul Qawee Mahyoob Rani, Kazumichi Fujioka, Hisahide Nishio, Masafumi Matsuo
Format: Article
Language:English
Published: MDPI AG 2020-11-01
Series:International Journal of Molecular Sciences
Subjects:
antisense oligonucleotide
minigene
exon skipping
splicing
dual fluorescence
<i>CD44</i>
Online Access:https://www.mdpi.com/1422-0067/21/23/9136

Internet

https://www.mdpi.com/1422-0067/21/23/9136

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