MAxSIM: multi-angle-crossing structured illumination microscopy with height-controlled mirror for 3D topological mapping of live cells

Abstract Mapping 3D plasma membrane topology in live cells can bring unprecedented insights into cell biology. Widefield-based super-resolution methods such as 3D-structured illumination microscopy (3D-SIM) can achieve twice the axial ( ~ 300 nm) and lateral ( ~ 100 nm) resolution of widefield micro...

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Bibliographic Details
Main Authors: Pedro Felipe Gardeazabal Rodriguez, Yigal Lilach, Abhijit Ambegaonkar, Teresa Vitali, Haani Jafri, Hae Won Sohn, Matthew Dalva, Susan Pierce, Inhee Chung
Format: Article
Language:English
Published: Nature Portfolio 2023-10-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-023-05380-2