MAxSIM: multi-angle-crossing structured illumination microscopy with height-controlled mirror for 3D topological mapping of live cells
Abstract Mapping 3D plasma membrane topology in live cells can bring unprecedented insights into cell biology. Widefield-based super-resolution methods such as 3D-structured illumination microscopy (3D-SIM) can achieve twice the axial ( ~ 300 nm) and lateral ( ~ 100 nm) resolution of widefield micro...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-10-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-023-05380-2 |
| _version_ | 1797557177937821696 |
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| author | Pedro Felipe Gardeazabal Rodriguez Yigal Lilach Abhijit Ambegaonkar Teresa Vitali Haani Jafri Hae Won Sohn Matthew Dalva Susan Pierce Inhee Chung |
| author_facet | Pedro Felipe Gardeazabal Rodriguez Yigal Lilach Abhijit Ambegaonkar Teresa Vitali Haani Jafri Hae Won Sohn Matthew Dalva Susan Pierce Inhee Chung |
| author_sort | Pedro Felipe Gardeazabal Rodriguez |
| collection | DOAJ |
| description | Abstract Mapping 3D plasma membrane topology in live cells can bring unprecedented insights into cell biology. Widefield-based super-resolution methods such as 3D-structured illumination microscopy (3D-SIM) can achieve twice the axial ( ~ 300 nm) and lateral ( ~ 100 nm) resolution of widefield microscopy in real time in live cells. However, twice-resolution enhancement cannot sufficiently visualize nanoscale fine structures of the plasma membrane. Axial interferometry methods including fluorescence light interference contrast microscopy and its derivatives (e.g., scanning angle interference microscopy) can determine nanoscale axial locations of proteins on and near the plasma membrane. Thus, by combining super-resolution lateral imaging of 2D-SIM with axial interferometry, we developed multi-angle-crossing structured illumination microscopy (MAxSIM) to generate multiple incident angles by fast, optoelectronic creation of diffraction patterns. Axial localization accuracy can be enhanced by placing cells on a bottom glass substrate, locating a custom height-controlled mirror (HCM) at a fixed axial position above the glass substrate, and optimizing the height reconstruction algorithm for noisy experimental data. The HCM also enables imaging of both the apical and basal surfaces of a cell. MAxSIM with HCM offers high-fidelity nanoscale 3D topological mapping of cell plasma membranes with near-real-time ( ~ 0.5 Hz) imaging of live cells and 3D single-molecule tracking. |
| first_indexed | 2024-03-10T17:13:40Z |
| format | Article |
| id | doaj.art-e998dbd23f8d4ebaaa48e5decf0bcf99 |
| institution | Directory Open Access Journal |
| issn | 2399-3642 |
| language | English |
| last_indexed | 2024-03-10T17:13:40Z |
| publishDate | 2023-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj.art-e998dbd23f8d4ebaaa48e5decf0bcf992023-11-20T10:34:49ZengNature PortfolioCommunications Biology2399-36422023-10-016111010.1038/s42003-023-05380-2MAxSIM: multi-angle-crossing structured illumination microscopy with height-controlled mirror for 3D topological mapping of live cellsPedro Felipe Gardeazabal Rodriguez0Yigal Lilach1Abhijit Ambegaonkar2Teresa Vitali3Haani Jafri4Hae Won Sohn5Matthew Dalva6Susan Pierce7Inhee Chung8Department of Anatomy and Cell Biology, George Washington University, School of Medicine and Health SciencesNanofabrication and Imaging Center, George Washington UniversityLaboratory of Immunogenetics, National Institute of Allergy and Infectious Disease, National Institutes of HealthDepartment of Anatomy and Cell Biology, George Washington University, School of Medicine and Health SciencesDepartment of Neuroscience, Thomas Jefferson UniversityLaboratory of Immunogenetics, National Institute of Allergy and Infectious Disease, National Institutes of HealthDepartment of Neuroscience, Thomas Jefferson UniversityLaboratory of Immunogenetics, National Institute of Allergy and Infectious Disease, National Institutes of HealthDepartment of Anatomy and Cell Biology, George Washington University, School of Medicine and Health SciencesAbstract Mapping 3D plasma membrane topology in live cells can bring unprecedented insights into cell biology. Widefield-based super-resolution methods such as 3D-structured illumination microscopy (3D-SIM) can achieve twice the axial ( ~ 300 nm) and lateral ( ~ 100 nm) resolution of widefield microscopy in real time in live cells. However, twice-resolution enhancement cannot sufficiently visualize nanoscale fine structures of the plasma membrane. Axial interferometry methods including fluorescence light interference contrast microscopy and its derivatives (e.g., scanning angle interference microscopy) can determine nanoscale axial locations of proteins on and near the plasma membrane. Thus, by combining super-resolution lateral imaging of 2D-SIM with axial interferometry, we developed multi-angle-crossing structured illumination microscopy (MAxSIM) to generate multiple incident angles by fast, optoelectronic creation of diffraction patterns. Axial localization accuracy can be enhanced by placing cells on a bottom glass substrate, locating a custom height-controlled mirror (HCM) at a fixed axial position above the glass substrate, and optimizing the height reconstruction algorithm for noisy experimental data. The HCM also enables imaging of both the apical and basal surfaces of a cell. MAxSIM with HCM offers high-fidelity nanoscale 3D topological mapping of cell plasma membranes with near-real-time ( ~ 0.5 Hz) imaging of live cells and 3D single-molecule tracking.https://doi.org/10.1038/s42003-023-05380-2 |
| spellingShingle | Pedro Felipe Gardeazabal Rodriguez Yigal Lilach Abhijit Ambegaonkar Teresa Vitali Haani Jafri Hae Won Sohn Matthew Dalva Susan Pierce Inhee Chung MAxSIM: multi-angle-crossing structured illumination microscopy with height-controlled mirror for 3D topological mapping of live cells Communications Biology |
| title | MAxSIM: multi-angle-crossing structured illumination microscopy with height-controlled mirror for 3D topological mapping of live cells |
| title_full | MAxSIM: multi-angle-crossing structured illumination microscopy with height-controlled mirror for 3D topological mapping of live cells |
| title_fullStr | MAxSIM: multi-angle-crossing structured illumination microscopy with height-controlled mirror for 3D topological mapping of live cells |
| title_full_unstemmed | MAxSIM: multi-angle-crossing structured illumination microscopy with height-controlled mirror for 3D topological mapping of live cells |
| title_short | MAxSIM: multi-angle-crossing structured illumination microscopy with height-controlled mirror for 3D topological mapping of live cells |
| title_sort | maxsim multi angle crossing structured illumination microscopy with height controlled mirror for 3d topological mapping of live cells |
| url | https://doi.org/10.1038/s42003-023-05380-2 |
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