Effect of seasonal variation on the chemical composition and antioxidant and antifungal activities of Convolvulus althaeoides L. leaf extracts
The composition of polyphenols, chlorophylls and carotenoids of eight extracts of Convolvulus althaeoides L. leaves, harvested in two different seasons, winter and spring, and extracted by hot extraction method using four solvents (dichloromethane, chloroform, ethyl acetate and ethanol) with increas...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-06-01
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| Series: | Arabian Journal of Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S187853522030112X |
| _version_ | 1819045446944817152 |
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| author | Soukaina Hrichi Raja Chaabane-Banaoues Daniele Giuffrida Domenica Mangraviti Yassine Oulad El Majdoub Francesca Rigano Luigi Mondello Hamouda Babba Zine Mighri Francesco Cacciola |
| author_facet | Soukaina Hrichi Raja Chaabane-Banaoues Daniele Giuffrida Domenica Mangraviti Yassine Oulad El Majdoub Francesca Rigano Luigi Mondello Hamouda Babba Zine Mighri Francesco Cacciola |
| author_sort | Soukaina Hrichi |
| collection | DOAJ |
| description | The composition of polyphenols, chlorophylls and carotenoids of eight extracts of Convolvulus althaeoides L. leaves, harvested in two different seasons, winter and spring, and extracted by hot extraction method using four solvents (dichloromethane, chloroform, ethyl acetate and ethanol) with increasing polarity, were evaluated along with their antioxidant and antifungal activities. Qualitative and quantitative variations were observed in the composition based on two different high performance liquid chromatography systems, liquid chromatography-photodiode array detection coupled to either atmospheric pressure chemical ionization mass spectrometry or to electrospray ionization mass spectrometry, permitting the identification of 22 polyphenols, 11 chlorophyll derivatives and 10 carotenoid compounds. Polyphenolic compounds were predominant in extracts from leaves collected in winter, whereas pigments were predominant in the spring collections. Antioxidant activities of the extracts were determined by DPPH radical scavenging method, revealing a half inhibition concentration (IC50) ranging from 0.1369 ± 0.0272 mg g−1 to 0.432 ± 0.0018 mg g−1, with no correlation to seasonal fluctuation. Concerning antifungal assays, ethyl acetate and ethanol extracts have been shown to be the most active against dermatophytes (T. rubrum, T. menthagrophytes, M. canis), with inhibiting percentages reaching 100% with 50 mg mL−1. Moreover, ethyl acetate and ethanol extracts showed a maximum inhibition potential with minimum inhibitory/fungicidal concentrations ranging from 0.78 to 6.25 mg mL−1 on Candida spp. cultures. The winter collect of these extracts showed an inhibitory effect of 90% on Candida albicans germ tubes formation, at a concentration of 3.1 mg mL−1. In conclusion, seasonality seems to influence the quality and the quantity of natural substances from leaves of C. althaeoides L., which have major importance on the antioxidant and the antifungal effectiveness. |
| first_indexed | 2024-12-21T10:28:43Z |
| format | Article |
| id | doaj.art-e9a5487ee9d44e8b82dfad40328b3467 |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-12-21T10:28:43Z |
| publishDate | 2020-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-e9a5487ee9d44e8b82dfad40328b34672022-12-21T19:07:15ZengElsevierArabian Journal of Chemistry1878-53522020-06-0113656515668Effect of seasonal variation on the chemical composition and antioxidant and antifungal activities of Convolvulus althaeoides L. leaf extractsSoukaina Hrichi0Raja Chaabane-Banaoues1Daniele Giuffrida2Domenica Mangraviti3Yassine Oulad El Majdoub4Francesca Rigano5Luigi Mondello6Hamouda Babba7Zine Mighri8Francesco Cacciola9Laboratory of Physico-Chemistry of Materials, Faculty of Sciences of Monastir, University of Monastir, Monastir, TunisiaLaboratory of Parasitology and Mycology (LP3M), Faculty of Pharmacy of Monastir, Department of Clinical Biology, University of Monastir, Monastir, TunisiaDepartment of Biomedical, Dental, Morphological and Functional Imaging Sciences, University of Messina, Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy; Chromaleont s.r.l., c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy; Department of Sciences and Technologies for Human and Environment, University Campus Bio-Medico of Rome, Rome, Italy; BeSep s.r.l., c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, ItalyLaboratory of Parasitology and Mycology (LP3M), Faculty of Pharmacy of Monastir, Department of Clinical Biology, University of Monastir, Monastir, TunisiaLaboratory of Physico-Chemistry of Materials, Faculty of Sciences of Monastir, University of Monastir, Monastir, TunisiaDepartment of Biomedical, Dental, Morphological and Functional Imaging Sciences, University of Messina, Messina, Italy; Corresponding author at: Department of Biomedical, Dental, Morphological and Functional Imaging Sciences, University of Messina, 98125 Messina, Italy.The composition of polyphenols, chlorophylls and carotenoids of eight extracts of Convolvulus althaeoides L. leaves, harvested in two different seasons, winter and spring, and extracted by hot extraction method using four solvents (dichloromethane, chloroform, ethyl acetate and ethanol) with increasing polarity, were evaluated along with their antioxidant and antifungal activities. Qualitative and quantitative variations were observed in the composition based on two different high performance liquid chromatography systems, liquid chromatography-photodiode array detection coupled to either atmospheric pressure chemical ionization mass spectrometry or to electrospray ionization mass spectrometry, permitting the identification of 22 polyphenols, 11 chlorophyll derivatives and 10 carotenoid compounds. Polyphenolic compounds were predominant in extracts from leaves collected in winter, whereas pigments were predominant in the spring collections. Antioxidant activities of the extracts were determined by DPPH radical scavenging method, revealing a half inhibition concentration (IC50) ranging from 0.1369 ± 0.0272 mg g−1 to 0.432 ± 0.0018 mg g−1, with no correlation to seasonal fluctuation. Concerning antifungal assays, ethyl acetate and ethanol extracts have been shown to be the most active against dermatophytes (T. rubrum, T. menthagrophytes, M. canis), with inhibiting percentages reaching 100% with 50 mg mL−1. Moreover, ethyl acetate and ethanol extracts showed a maximum inhibition potential with minimum inhibitory/fungicidal concentrations ranging from 0.78 to 6.25 mg mL−1 on Candida spp. cultures. The winter collect of these extracts showed an inhibitory effect of 90% on Candida albicans germ tubes formation, at a concentration of 3.1 mg mL−1. In conclusion, seasonality seems to influence the quality and the quantity of natural substances from leaves of C. althaeoides L., which have major importance on the antioxidant and the antifungal effectiveness.http://www.sciencedirect.com/science/article/pii/S187853522030112XConvolvulusPolyphenolsCarotenoidsLiquid chromatographyAntioxidant activityAntifungal activity |
| spellingShingle | Soukaina Hrichi Raja Chaabane-Banaoues Daniele Giuffrida Domenica Mangraviti Yassine Oulad El Majdoub Francesca Rigano Luigi Mondello Hamouda Babba Zine Mighri Francesco Cacciola Effect of seasonal variation on the chemical composition and antioxidant and antifungal activities of Convolvulus althaeoides L. leaf extracts Arabian Journal of Chemistry Convolvulus Polyphenols Carotenoids Liquid chromatography Antioxidant activity Antifungal activity |
| title | Effect of seasonal variation on the chemical composition and antioxidant and antifungal activities of Convolvulus althaeoides L. leaf extracts |
| title_full | Effect of seasonal variation on the chemical composition and antioxidant and antifungal activities of Convolvulus althaeoides L. leaf extracts |
| title_fullStr | Effect of seasonal variation on the chemical composition and antioxidant and antifungal activities of Convolvulus althaeoides L. leaf extracts |
| title_full_unstemmed | Effect of seasonal variation on the chemical composition and antioxidant and antifungal activities of Convolvulus althaeoides L. leaf extracts |
| title_short | Effect of seasonal variation on the chemical composition and antioxidant and antifungal activities of Convolvulus althaeoides L. leaf extracts |
| title_sort | effect of seasonal variation on the chemical composition and antioxidant and antifungal activities of convolvulus althaeoides l leaf extracts |
| topic | Convolvulus Polyphenols Carotenoids Liquid chromatography Antioxidant activity Antifungal activity |
| url | http://www.sciencedirect.com/science/article/pii/S187853522030112X |
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