Phenolic profiles and biological properties of traditional Phyllanthus amarus aqueous extracts used for diabetes
For the first time, a complete study, including the fresh and dried aerial parts of Phyllanthus amarus was performed using Guadeloupe's population traditional extraction methods. In this study, the antidiabetic and antioxidant activities of P. amarus infusion and decoction in water with short b...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-08-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464621002206 |
| _version_ | 1818449061639880704 |
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| author | Mélissa Matou Sylvie Bercion Thérèse Marianne-Pepin Pierre Haddad Patrick Merciris |
| author_facet | Mélissa Matou Sylvie Bercion Thérèse Marianne-Pepin Pierre Haddad Patrick Merciris |
| author_sort | Mélissa Matou |
| collection | DOAJ |
| description | For the first time, a complete study, including the fresh and dried aerial parts of Phyllanthus amarus was performed using Guadeloupe's population traditional extraction methods. In this study, the antidiabetic and antioxidant activities of P. amarus infusion and decoction in water with short boiling time, were evaluated using glucose-6-phosphatase enzyme in H4IIE hepatic cells inhibition and stimulation of glucose uptake in C2C12 skeletal muscle cells, as well as using 2,2-diphenyl-1picrylhydrazyl (DPPH) radical scavenging activity and ferric reducing antioxidant power assays. A compound, caffeoyl hexaric acid, was tentatively identified for the first time in P. amarus extracts, using UHPLC-HRMS. Only dried aerial parts extracts exhibited strong stimulation of glucose uptake similar to that of insulin with an increase of 23%. The infusion of fresh material appeared to be the best way for aerial parts maximal anti-hyperglycemic activity with 55.8 ± 2.61% of glucose-6-phosphatase inhibition. |
| first_indexed | 2024-12-14T20:29:25Z |
| format | Article |
| id | doaj.art-e9bf3abc54db4752a872304533ed8b0e |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-14T20:29:25Z |
| publishDate | 2021-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-e9bf3abc54db4752a872304533ed8b0e2022-12-21T22:48:34ZengElsevierJournal of Functional Foods1756-46462021-08-0183104571Phenolic profiles and biological properties of traditional Phyllanthus amarus aqueous extracts used for diabetesMélissa Matou0Sylvie Bercion1Thérèse Marianne-Pepin2Pierre Haddad3Patrick Merciris4UMR 1270 QUALITROP, Université des Antilles, Campus de Fouillole, BP 250 97157 Pointe à Pitre Cedex (French West Indies), France; EA 7525 Vulnérabilité Cardiovasculaire, Pathologie métabolique et endocrinienne, Université des Antilles, Campus de Fouillole, BP 250 97157 Pointe à Pitre Cedex (French West Indies), France; Corresponding authors at: EA 7525 Vulnérabilité Cardiovasculaire, Pathologie métabolique et endocrinienne, Université des Antilles, Campus de Fouillole, BP 250 97157 Pointe à Pitre Cedex (French West Indies), France.UMR 1270 QUALITROP, Université des Antilles, Campus de Fouillole, BP 250 97157 Pointe à Pitre Cedex (French West Indies), France; EA 7525 Vulnérabilité Cardiovasculaire, Pathologie métabolique et endocrinienne, Université des Antilles, Campus de Fouillole, BP 250 97157 Pointe à Pitre Cedex (French West Indies), France; Corresponding authors at: EA 7525 Vulnérabilité Cardiovasculaire, Pathologie métabolique et endocrinienne, Université des Antilles, Campus de Fouillole, BP 250 97157 Pointe à Pitre Cedex (French West Indies), France.UMR 1270 QUALITROP, Université des Antilles, Campus de Fouillole, BP 250 97157 Pointe à Pitre Cedex (French West Indies), France; Department of Biology– Université des Antilles, Campus de Fouillole, BP 250 97157 Pointe à Pitre Cedex (French West Indies), FranceNatural Health Products and Metabolic Diseases Laboratory, Department of Pharmacology and Physiology, Université de Montréal, Montreal, Quebec H3C 3J7, CanadaUMR 1270 QUALITROP, Université des Antilles, Campus de Fouillole, BP 250 97157 Pointe à Pitre Cedex (French West Indies), France; Department of Biology– Université des Antilles, Campus de Fouillole, BP 250 97157 Pointe à Pitre Cedex (French West Indies), France; Corresponding authors at: EA 7525 Vulnérabilité Cardiovasculaire, Pathologie métabolique et endocrinienne, Université des Antilles, Campus de Fouillole, BP 250 97157 Pointe à Pitre Cedex (French West Indies), France.For the first time, a complete study, including the fresh and dried aerial parts of Phyllanthus amarus was performed using Guadeloupe's population traditional extraction methods. In this study, the antidiabetic and antioxidant activities of P. amarus infusion and decoction in water with short boiling time, were evaluated using glucose-6-phosphatase enzyme in H4IIE hepatic cells inhibition and stimulation of glucose uptake in C2C12 skeletal muscle cells, as well as using 2,2-diphenyl-1picrylhydrazyl (DPPH) radical scavenging activity and ferric reducing antioxidant power assays. A compound, caffeoyl hexaric acid, was tentatively identified for the first time in P. amarus extracts, using UHPLC-HRMS. Only dried aerial parts extracts exhibited strong stimulation of glucose uptake similar to that of insulin with an increase of 23%. The infusion of fresh material appeared to be the best way for aerial parts maximal anti-hyperglycemic activity with 55.8 ± 2.61% of glucose-6-phosphatase inhibition.http://www.sciencedirect.com/science/article/pii/S1756464621002206Phyllanthus amarusInfusionDecoctionPolyphenolsAntidiabetic activityAntioxidant activity |
| spellingShingle | Mélissa Matou Sylvie Bercion Thérèse Marianne-Pepin Pierre Haddad Patrick Merciris Phenolic profiles and biological properties of traditional Phyllanthus amarus aqueous extracts used for diabetes Journal of Functional Foods Phyllanthus amarus Infusion Decoction Polyphenols Antidiabetic activity Antioxidant activity |
| title | Phenolic profiles and biological properties of traditional Phyllanthus amarus aqueous extracts used for diabetes |
| title_full | Phenolic profiles and biological properties of traditional Phyllanthus amarus aqueous extracts used for diabetes |
| title_fullStr | Phenolic profiles and biological properties of traditional Phyllanthus amarus aqueous extracts used for diabetes |
| title_full_unstemmed | Phenolic profiles and biological properties of traditional Phyllanthus amarus aqueous extracts used for diabetes |
| title_short | Phenolic profiles and biological properties of traditional Phyllanthus amarus aqueous extracts used for diabetes |
| title_sort | phenolic profiles and biological properties of traditional phyllanthus amarus aqueous extracts used for diabetes |
| topic | Phyllanthus amarus Infusion Decoction Polyphenols Antidiabetic activity Antioxidant activity |
| url | http://www.sciencedirect.com/science/article/pii/S1756464621002206 |
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