Insight into the molecular mechanism of the transposon-encoded type I-F CRISPR-Cas system

Abstract CRISPR-Cas9 is a popular gene-editing tool that allows researchers to introduce double-strand breaks to edit parts of the genome. CRISPR-Cas9 system is used more than other gene-editing tools because it is simple and easy to customize. However, Cas9 may produce unintended double-strand brea...

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Bibliographic Details
Main Authors: Amnah Alalmaie, Saousen Diaf, Raed Khashan
Format: Article
Language:English
Published: SpringerOpen 2023-05-01
Series:Journal of Genetic Engineering and Biotechnology
Subjects:
Online Access:https://doi.org/10.1186/s43141-023-00507-8